College of Resources and Environment, Northwest A&F University, Yangling 712100, P R China.
College of Environment, Hohai University, Nanjing 210098, P R China.
Sci Rep. 2017 Jan 12;7:40393. doi: 10.1038/srep40393.
Copper sulfate is a frequently used reagent for Microcystis blooms control but almost all the previous works have used Microcystis aeruginosa as the target organism to determine dosages. The aim of this study was to evaluate interspecific differences in the responses of various Microcystis species to varying Cu concentrations (0, 0.05, 0.10, 0.25, and 0.50 mg L). The half maximal effective concentration values for M. aeruginosa, M. wesenbergii, M. flos-aquae, and M. viridis were 0.16, 0.09, 0.49, and 0.45 mg L Cu, respectively. This showed a species-dependent variation in the sensitivity of Microcystis species to copper sulfate. Malonaldehyde content did not decrease with increasing superoxide dismutase content induced by increasing Cu, suggesting that superoxide dismutase failed to reduce Cu damage in Microcystis. Considering the risk of microcystin release when Microcystis membranes are destroyed as a result of Cu treatment and the stimulation effects of a low level of Cu on growth in various species, our results suggest that copper sulfate treatment for Microcystis control could be applied before midsummer when M. aeruginosa and M. viridis are not the dominant species and actual amount of Cu used to control M. wesenbergii should be much greater than 0.10 mg L.
硫酸铜是一种常用于控制微囊藻水华的试剂，但几乎所有以前的工作都使用铜绿微囊藻作为目标生物来确定剂量。本研究旨在评估不同种微囊藻对不同铜浓度（0、0.05、0.10、0.25 和 0.50mg/L）的反应的种间差异。铜绿微囊藻、摩西管形藻、水华鱼腥藻和绿色微囊藻的半最大有效浓度值分别为 0.16、0.09、0.49 和 0.45mg/L Cu。这表明微囊藻对硫酸铜的敏感性存在种依赖性变化。随着 Cu 诱导的超氧化物歧化酶含量的增加，丙二醛含量并没有降低，这表明超氧化物歧化酶未能减轻 Cu 对微囊藻的损伤。考虑到 Cu 处理导致微囊藻膜破坏时释放微囊藻毒素的风险，以及低水平 Cu 对各种物种生长的刺激作用，我们的研究结果表明，在中夏之前，可以应用硫酸铜处理来控制微囊藻，此时铜绿微囊藻和绿色微囊藻不是优势物种，用于控制摩西管形藻的实际 Cu 用量应该远大于 0.10mg/L。
