Sellheyer Klaus, Krahl Dieter
Department of Dermatology, Cleveland Clinic Foundation, Cleveland, OH, USA.
J Cutan Pathol. 2010 Mar;37(3):357-61. doi: 10.1111/j.1600-0560.2009.01416.x. Epub 2009 Aug 27.
The transcription factor GATA-3 was recently identified as a master regulator in the specification of the inner root sheath. Additionally, it seems to play a role in skin barrier physiology. p63 binds and transactivates the GATA-3 promoter. While the expression profile of GATA-3 is delineated for the mouse, little is known about its expression in the adult human hair follicle and no studies are published about its distribution during human cutaneous embryogenesis.
We examined samples from embryonic, fetal and adult human skin for the expression of GATA-3 using immunohistochemistry.
GATA-3 is expressed late during human skin development. Its expression pattern is comparable to the mouse and confined to the Huxley layer and inner root sheath cuticle but sparing the Henle layer. In addition, GATA-3 localizes to the spinous cell layer of the interfollicular epidermis.
From the described expression pattern, it is highly probable that GATA-3 plays a role in follicular and epidermal morphogenesis. What the anatomically confined expression of GATA-3 to the spinous layer means biologically for the physiology of the skin is still unclear. Likewise, it still needs to be shown if GATA-3 could be exploited in the diagnosis of adnexal neoplasms.
转录因子GATA-3最近被确定为内根鞘形成过程中的主要调节因子。此外，它似乎在皮肤屏障生理中发挥作用。p63结合并反式激活GATA-3启动子。虽然已明确了GATA-3在小鼠中的表达谱，但对其在成人毛囊中的表达了解甚少，且尚无关于其在人类皮肤胚胎发生过程中分布的研究报道。
我们使用免疫组织化学方法检测了来自人类胚胎、胎儿和成人皮肤的样本中GATA-3的表达情况。
GATA-3在人类皮肤发育后期表达。其表达模式与小鼠相似，局限于赫胥黎层和内根鞘角质层，但亨勒层无表达。此外，GATA-3定位于毛囊间表皮的棘细胞层。
从所描述的表达模式来看，GATA-3极有可能在毛囊和表皮形态发生中发挥作用。GATA-3在解剖学上局限于棘层的表达对皮肤生理的生物学意义仍不清楚。同样，GATA-3是否可用于附属器肿瘤的诊断仍有待证实。
