Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China.
Int J Mol Sci. 2022 Sep 2;23(17):10006. doi: 10.3390/ijms231710006.
Cashmere is initiated and develops in the fetal stages and the number and density of secondary hair follicles (SHFs) determine cashmere production and quality. Growing evidence indicates that both microRNA (miRNA) and long non-coding RNA (lncRNA) play an indispensable role in hair follicle (HF) growth and development. However, little is known about miRNAs, lncRNAs, and their functions as well as their interactions during cashmere initiation and development. Here, based on lncRNA and miRNA high-throughput sequencing and bioinformatics analysis, we identified 10,485 lncRNAs, 40,639 mRNAs, and 605 miRNAs in cashmere goat skin during HF induction, organogenesis, and cytodifferentiation stages. Among them, 521 lncRNAs, 5976 genes, and 204 miRNAs were differentially expressed (DE). KEGG analysis of DE genes indicated that ECM-receptor interaction and biosynthesis of amino acids were crucial for HF development. Notch, TGF-beta, and Wnt signaling pathways were also identified, which are conventional pathways associated with HF growth and development. Then, the ceRNA regulatory network was constructed, and the impact of lncRNA H19 was investigated in dermal papilla (DP) cells. The MTT, CCK-8, and EdU assays showed that the viability and proliferation of DP cells were promoted by H19, and mechanistic studies suggested that H19 performed its function through the chi-miR-214-3p/β-catenin axis. The present study created a resource for lncRNA, miRNA, and mRNA studies in cashmere morphogenesis. It could contribute to a better understanding of the molecular mechanism of ncRNAs involved in the regulation of HF growth and development.
羊绒是在胎儿期开始形成和发育的，次级毛囊（SHF）的数量和密度决定了羊绒的产量和质量。越来越多的证据表明，微小 RNA（miRNA）和长非编码 RNA（lncRNA）在毛囊（HF）生长和发育中起着不可或缺的作用。然而，对于 miRNA、lncRNA 及其在羊绒起始和发育过程中的功能及其相互作用知之甚少。在这里，基于 lncRNA 和 miRNA 高通量测序和生物信息学分析，我们在 HF 诱导、器官发生和细胞分化阶段鉴定了绒山羊皮肤中的 10485 个 lncRNA、40639 个 mRNA 和 605 个 miRNA。其中，521 个 lncRNA、5976 个基因和 204 个 miRNA 差异表达（DE）。DE 基因的 KEGG 分析表明，ECM-受体相互作用和氨基酸生物合成对 HF 发育至关重要。还鉴定了 Notch、TGF-β和 Wnt 信号通路，这些通路是与 HF 生长和发育相关的常规通路。然后构建了 ceRNA 调控网络，并研究了 lncRNA H19 在真皮乳头（DP）细胞中的作用。MTT、CCK-8 和 EdU 检测表明 H19 促进 DP 细胞的活力和增殖，机制研究表明 H19 通过 chi-miR-214-3p/β-catenin 轴发挥其功能。本研究为绒山羊形态发生中的 lncRNA、miRNA 和 mRNA 研究创建了一个资源库，有助于更好地理解 ncRNA 参与调控 HF 生长和发育的分子机制。
