Sun Wen-jing, Qin Bo, Huang Tao, Huang Ai-long
Department of Infection Disease, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Zhonghua Gan Zang Bing Za Zhi. 2009 Aug;17(8):615-9.
To investigate the effect of Hyper-IL-6 on the proliferation of L-02 cells.
The recombinant lentiviral vectors encoding Hyper-IL-6 (FIV-Hyper-IL-6), IL-6 (FIV-IL-6) and the lentiviral (FIV) were prepared using the FIV-based lentiviral vectors and the packaging system. The titer of FIV-Hyper-IL-6, FIV-IL-6 and FIV was tested with puromycin. L-02 cells were infected with FIV-Hyper-IL-6, FIV-IL-6, FIV, or mock-infected. The growth rate of L02 cells was analyzed with MTT at different time points after infection. The changes of Haptoglobin in L-02 cells were analyzed with RT-PCR.
FIV-Hyper-IL-6, FIV-IL-6 and FIV were successfully constructed, and the titer was 107 pfu/ml. 48 hours after infection, the absorbance of the cells infected with FIV-Hyper-IL-6 was 0.6267+/-0.0256, and the absorbances of FIV-IL-6 infected cells, FIV infected cells and mock-infected cells were 0.5563+/-0.0112, 0.5040+/-0.0078 and 0.4790+/-0.0201, respectively. There were significant differences between the FIV-Hyper-IL-6 group and the other groups (F = 41.09, P less than 0.01). The ratio of the absorbance between haptoglobin mRNA and beta-actin was 0.7030+/-0.0106, 0.3355+/-0.0093, 0.1145+/-0.0076 and 0.1143+/-0.0153, respectively, in FIV-Hyper-IL-6 infected cells, FIV-IL-6 infected cells, FIV infected cells and mock-infected cells. There were significant differences between the FIV-Hyper-IL-6 group and the others (q = 57.5007, P less than 0.01).
Compared with IL-6, Hyper-IL-6 is more potent to stimulate proliferation and induce the expression of Haptoglobin in L-02 cells.
研究超白细胞介素-6（Hyper-IL-6）对L-02细胞增殖的影响。
使用基于泡沫病毒（FIV）的慢病毒载体和包装系统制备编码Hyper-IL-6（FIV-Hyper-IL-6）、白细胞介素-6（IL-6，FIV-IL-6）的重组慢病毒载体以及慢病毒（FIV）。用嘌呤霉素检测FIV-Hyper-IL-6、FIV-IL-6和FIV的滴度。L-02细胞分别用FIV-Hyper-IL-6、FIV-IL-6、FIV感染或进行mock感染。感染后不同时间点用MTT分析L02细胞的生长速率。用RT-PCR分析L-02细胞中触珠蛋白的变化。
成功构建FIV-Hyper-IL-6、FIV-IL-6和FIV，滴度为107 pfu/ml。感染后48小时，FIV-Hyper-IL-6感染的细胞吸光度为0.6267±0.0256，FIV-IL-6感染的细胞、FIV感染的细胞和mock感染的细胞吸光度分别为0.5563±0.0112、0.5040±0.0078和0.4790±0.0201。FIV-Hyper-IL-6组与其他组之间存在显著差异（F = 41.09，P＜0.01）。FIV-Hyper-IL-6感染的细胞、FIV-IL-6感染的细胞、FIV感染的细胞和mock感染的细胞中触珠蛋白mRNA与β-肌动蛋白吸光度之比分别为0.7030±0.0106、0.3355±0.0093、0.1145±0.0076和0.1143±0.0153。FIV-Hyper-IL-6组与其他组之间存在显著差异（q = 57.5007，P＜0.01）。
与IL-6相比，Hyper-IL-6更能有效刺激L-02细胞增殖并诱导触珠蛋白表达。
