中药参松养心（SSYX）对中国小型猪心脏和离体豚鼠心室肌细胞的电生理效应。

Electrophysiological effects of Chinese medicine Shen song Yang xin (SSYX) on Chinese miniature swine heart and isolated guinea pig ventricular myocytes.

作者信息

Feng Li, Gong Jing, Jin Zhen-yi, Li Ning, Sun Li-ping, Wu Yi-ling, Pu Jie-lin

机构信息

Center for Arrhythmia Diagnosis and Treatment, Fuwai Cardiovascular Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China.

出版信息

Chin Med J (Engl). 2009 Jul 5;122(13):1539-43.

PMID:19719944

Abstract

BACKGROUND

Shen song Yang xin (SSYX) is a compound of Chinese medicine with the effect of increasing heart rate (HR). This study aimed to evaluate its electrophysiological properties at heart and cellular levels.

METHODS

The Chinese miniature swines were randomly assigned to two groups, administered with SSYX or placebo for 4 weeks (n = 8 per group). Cardiac electrophysiological study (EPS) was performed before and after drug administration. The guinea pig ventricular myocytes were enzymatically isolated and whole cell voltage-clamp technique was used to evaluate the effect of SSYX on cardiac action potential (AP).

RESULTS

SSYX treatment accelerated the HR from (141.8 +/- 36.0) beats per minute to (163.0 +/- 38.0) beats per minute (P = 0.013) without changing the other parameters in surface electrocardiogram. After blockage of the autonomic nervous system with metoprolol and atropin, SSYX had no effect on intrinsic HR (IHR), but decreased corrected sinus node recovery time (CSNRT) and sinus atrium conducting time (SACT). Intra cardiac EPS showed that SSYX significantly decreased the A-H and A-V intervals as well as shortened the atrial (A), atrioventricular node (AVN) and ventricular (V) effective refractory period (ERP). In isolated guinea pig ventricular myocytes, the most obvious effect of SSYX on action potential was a shortening of the action potential duration (APD) without change in shape of action potential. The shortening rates of APD(30), APD(50) and APD(90) were 19.5%, 17.8% and 15.3%, respectively. The resting potential (Em) and the interval between the end of APD(30) and APD(90) did not significantly change.

CONCLUSIONS

The present study demonstrates that SSYX increases the HR and enhances the conducting capacity of the heart in the condition of the intact autonomic nervous system. SSYX homogenously decreases the ERP of the heart and shortens the APD of the myocytes, suggesting its antiarrhythmic effect without proarrhythmia.

摘要

背景

参松养心（SSYX）是一种具有提高心率（HR）作用的中药复方。本研究旨在评估其在心脏和细胞水平的电生理特性。

方法

将中国小型猪随机分为两组，分别给予参松养心或安慰剂4周（每组n = 8）。在给药前后进行心脏电生理研究（EPS）。酶法分离豚鼠心室肌细胞，采用全细胞电压钳技术评估参松养心对心脏动作电位（AP）的影响。

结果

参松养心治疗使心率从（141.8±36.0）次/分钟加快至（163.0±38.0）次/分钟（P = 0.013），而体表心电图的其他参数未改变。用美托洛尔和阿托品阻断自主神经系统后，参松养心对固有心率（IHR）无影响，但缩短了校正窦房结恢复时间（CSNRT）和窦房传导时间（SACT）。心内EPS显示，参松养心显著缩短A-H和A-V间期，并缩短心房（A）、房室结（AVN）和心室（V）的有效不应期（ERP）。在分离的豚鼠心室肌细胞中，参松养心对动作电位最明显的影响是缩短动作电位时程（APD），而动作电位形状无改变。APD(30)、APD(50)和APD(90)的缩短率分别为19.5%、17.8%和15.3%。静息电位（Em）以及APD(30)结束至APD(90)的间期无显著变化。