Delorenzi M, Bienz M
Zoological Institute, University of Zürich, Switzerland.
Development. 1990 Feb;108(2):323-9. doi: 10.1242/dev.108.2.323.
The Abdominal-B (Abd-B) gene determines development of the posteriormost segments in Drosophila. Genetic and molecular analysis suggested that it consists of two genetically separable functions that are conferred by two related homeoproteins termed m and r. We have raised an antiserum against Abd-B protein to describe the patterns of Abd-B protein expression during embryonic development. The pattern of r protein expression, as deduced by analysis of Abd-B mutants, is restricted to ps14 and 15 in all germ layers and observes a parasegmental boundary at its anterior margin of expression. In contrast, the pattern of m protein expression is unusual as its level in the ectoderm increases from ps10 to ps13 in parasegmental steps. Its anterior margin of expression is highly dynamic shifting anteriorly across more than 3 parasegments during midembryonic development. Evidently, the control mechanisms of m and r protein expression are considerably different. Moreover, an antibody-positive Abd-B mutant suggests that these differ, in the case of m protein expression, to some extent in individual germ layers.
腹部B（Abd - B）基因决定果蝇最末段的发育。遗传和分子分析表明，它由两个遗传上可分离的功能组成，这两个功能由两种相关的同源蛋白m和r赋予。我们制备了一种针对Abd - B蛋白的抗血清，以描述胚胎发育过程中Abd - B蛋白的表达模式。通过对Abd - B突变体的分析推断，r蛋白的表达模式在所有胚层中都局限于副节14和15，并且在其表达的前缘观察到一个副节边界。相比之下，m蛋白的表达模式不同寻常，因为其在外胚层中的水平在副节步骤中从副节10增加到副节13。其表达的前缘高度动态，在胚胎发育中期向前移动超过3个副节。显然，m和r蛋白表达的控制机制有很大差异。此外，一个抗体阳性的Abd - B突变体表明，就m蛋白表达而言，这些差异在个别胚层中在一定程度上存在。
