Department of Food Science and Technology, CEBAS (CSIC), Murcia, Spain.
J Pharm Biomed Anal. 2010 Jan 5;51(1):65-9. doi: 10.1016/j.jpba.2009.08.005. Epub 2009 Aug 12.
Catharanthus roseus is one of the most important medicinal plants worldwide. The leaves of this species are the only source of the indolomonoterpenic alkaloids vincristin (leurocristine) and vinblastin (vincaleucoblastine), whose anticancer activity represents powerful therapeutics to many diseases, such as Hodgkin lymphoma. Usually, the remaining plant parts go to waste. Here we describe a phytochemical study on this species roots. Alkaloids in aqueous extracts, the usual form of consumption of this matrix, were studied using HPLC-DAD-ESI-MS/MS, which allowed the identification of 19-S-vindolinine, vindolinine, ajmalicine and an ajmalicine isomer, tabersonine, catharanthine, serpentine and a serpentine isomer. Quantification of the identified compounds revealed that serpentine and its isomer were predominant (64.7%) over the other alkaloids, namely vindolinine and its isomer (23.9%), catharanthine (7.7%) and ajmalicine (3.8%). The used procedure revealed to be simple, sensitive and reproducible.
长春花是世界上最重要的药用植物之一。该物种的叶子是吲哚单萜生物碱长春新碱（leurocristine）和长春碱（vincaleucoblastine）的唯一来源，其抗癌活性对许多疾病具有强大的治疗作用，如霍奇金淋巴瘤。通常，其余的植物部分都会被浪费掉。在这里，我们描述了对该物种根的植物化学研究。使用 HPLC-DAD-ESI-MS/MS 研究了水提物中的生物碱，这是该基质的常用形式，该方法鉴定出 19-S- vindolinine、vindolinine、ajmalicine 和 ajmalicine 异构体、tabersonine、长春碱、蛇根碱和蛇根碱异构体。鉴定化合物的定量分析表明，蛇根碱及其异构体（64.7%）明显高于其他生物碱，即 vindolinine 及其异构体（23.9%）、长春碱（7.7%）和 ajmalicine（3.8%）。所用方法简单、灵敏且重现性好。
