Abdul Rahim Rohanizah, Ahmad Nor Hazwani, Al Azzam Khaldun Mohammad, Mat Ishak
Advanced Medical & Dental Institute, Universiti Sains Malaysia, 13200 Kepala Batas, Pulau Pinang, Malaysia.
Preparatory Year Department, Al-Ghad International Colleges for Applied Medical Sciences, Riyadh, Kingdom of Saudi Arabia.
Adv Pharm Bull. 2018 Mar;8(1):157-161. doi: 10.15171/apb.2018.019. Epub 2018 Mar 18.
To determine and quantify vinblastine in different varieties of Catharanthus roseus using reversed-phase HPLC method. The liquid chromatographic separation was performed using a reversed phase C18, Microsorb - MV column (250 mm x 4.6 mm, 5 µm) at room temperature and eluted with a mobile phase containing methanol - phosphate buffer (5 mM, pH 6.0) - acetonitrile with different proportion gradient elution at a flow rate of 2.0 mL min and detection at 254 nm. The HPLC method was utilized for the quantification of vinblastine in purple, red and white varieties of Catharanthus roseus leaves. The separation was achieved in less than 8 min. The peak confirmation was done based on the retention times and UV spectra of the reference substance. The method was validated with respect to linearity, precision, recovery, limit of detection and quantification. Results showed that the purple variety gives 1.2 and 1.5 times more vinblastine concentration compared to the white and pink varieties, respectively. The obtained results from different varieties are thus useful for the purpose of vinblastine production from Catharanthus roseus plant.
采用反相高效液相色谱法测定并定量分析不同品种长春花中长春碱的含量。液相色谱分离在室温下使用反相C18、Microsorb - MV柱（250 mm×4.6 mm，5 µm）进行，以含有甲醇 - 磷酸盐缓冲液（5 mM，pH 6.0） - 乙腈的流动相以不同比例梯度洗脱，流速为2.0 mL/min，检测波长为254 nm。该高效液相色谱法用于定量分析长春花紫色、红色和白色品种叶片中的长春碱。分离在不到8分钟内完成。根据对照品的保留时间和紫外光谱进行峰确认。该方法在线性、精密度、回收率、检测限和定量限方面进行了验证。结果表明，紫色品种长春碱浓度分别比白色和粉色品种高1.2倍和1.5倍。因此，从不同品种获得的结果对于从长春花植物中生产长春碱具有重要意义。
