Aiken J M, Williamson J L, Borchardt L M, Marsh R F
Department of Veterinary Science, University of Wisconsin-Madison 53706.
J Virol. 1990 Jul;64(7):3265-8. doi: 10.1128/JVI.64.7.3265-3268.1990.
The prion preparation has, in recent years, been the focal point of scrapie research. The inability to identify agent-specific nucleic acids in this sample has led to the formulation of the infectious protein or prion hypothesis. In this study, we analyzed three different prion protein-enriched preparations and found all to contain significant amounts of mitochondrial nucleic acid. Southern blot analyses indicated that they are enriched for a specific component of the mitochondrial genome, the single-stranded displacement loop fragment. Our results suggest that if mitochondrial nucleic acids are involved in scrapie infection, it is the displacement loop fragment that is specifically responsible.
近年来，朊病毒制剂一直是羊瘙痒病研究的焦点。由于无法在该样本中鉴定出病原体特异性核酸，导致了感染性蛋白质或朊病毒假说的形成。在本研究中，我们分析了三种不同的富含朊病毒蛋白的制剂，发现它们都含有大量的线粒体核酸。Southern印迹分析表明，它们富含线粒体基因组的一个特定成分，即单链置换环片段。我们的结果表明，如果线粒体核酸参与了羊瘙痒病感染，那么特异性起作用的就是置换环片段。
