Lisby G
Department of Clinical Microbiology, Herler Hospital, Copenhagen, Denmark.
Mol Biotechnol. 1999 Aug;12(1):75-99. doi: 10.1385/MB:12:1:75.
The use of nucleic acid amplification methods in routine clinical microbiology laboratories is becoming increasingly widespread. The theory of polymerase chain reaction is described, including discussion of suitable microbal targets, extraction of nucleic acid from clinical samples, choice of primers, optimization of the process, laboratory design, contamination, and other problems as well as quality control. Other nucleic acid amplification methods such as ligase chain reaction, self-sustained sequence replication, strand displacement amplification, and branched DNA signal amplification are described and the choice of technology is discussed.
核酸扩增方法在常规临床微生物实验室中的应用正日益广泛。本文描述了聚合酶链反应的原理，包括对合适微生物靶标的讨论、从临床样本中提取核酸、引物的选择、该过程的优化、实验室设计、污染及其他问题以及质量控制。还描述了其他核酸扩增方法，如连接酶链反应、自我维持序列复制、链置换扩增和分支DNA信号扩增，并对技术选择进行了讨论。
