Department of Oral and Maxillofacial Surgery, Graduate School of Dentistry, Osaka University, 1-8 Yamada-oka, Suita, Osaka 565-0871, Japan.
J Biomed Mater Res A. 2010 Jun 1;93(3):965-75. doi: 10.1002/jbm.a.32518.
The main objective of this study was to evaluate the biological behavior of Hydroxyapatite (HAp)/agarose and calcium carbonate (CaCO3)/agarose composite gels by an alternate soaking process used for the treatment of surgically produced bone defects in rat cranium. We designed the following four groups: (i) HAp (HAp/agarose composite gel), (ii) CaCO3 (CaCO3/agarose composite gel), (iii) Agarose (bare agarose gel), and (iv) Defect (no filling materials). We subdivided (i) (ii) (iii) into two application types as a (I) Homogenized Group (homogenized materials) and a (II) Disk Group (disk shaped materials). We assessed samples by radiological and histological analyses 0, 4, and 8 weeks after implantation. The results indicated that the composite gels showed higher radiopacity in microfocus-computed tomography (muCT) images and showed higher volume in quantitative analyses using Dual Energy X-ray Absorptiometry (DEXA) and Peripheral Quantitative Computed Tomography (pQCT) than the Agarose and Defect groups. The histological examination showed characteristic images due to each application form. Consequently, HAp and CaCO3/agarose composite gels can be expected to accelerate the speed of producing more new bone associated with osteogenesis. These novel biomaterials play an important role as an alternative biocompatible and biodegradable bone grafting filler material for autogenous bone.
本研究的主要目的是通过交替浸泡法评估羟磷灰石（HAp）/琼脂糖和碳酸钙（CaCO3）/琼脂糖复合凝胶的生物学行为，该方法用于治疗大鼠颅骨手术产生的骨缺损。我们设计了以下四个组：（i）HAp（HAp/琼脂糖复合凝胶），（ii）CaCO3（CaCO3/琼脂糖复合凝胶），（iii）琼脂糖（裸琼脂糖凝胶）和（iv）缺损（无填充材料）。我们将（i）（ii）（iii）进一步细分为（I）均质组（均质材料）和（II）圆盘组（圆盘状材料）。我们在植入后 0、4 和 8 周通过放射学和组织学分析评估样本。结果表明，复合凝胶在微焦点计算机断层扫描（microfocus-CT，muCT）图像中具有更高的放射密度，并在双能 X 射线吸收仪（DEXA）和外周定量计算机断层扫描（pQCT）的定量分析中显示出比琼脂糖和缺损组更高的体积。组织学检查显示出每种应用形式的特征图像。因此，HAp 和 CaCO3/琼脂糖复合凝胶有望加速与成骨相关的新骨的生成速度。这些新型生物材料在作为自体骨的替代生物相容性和可生物降解的骨移植填充材料方面发挥着重要作用。
