Department of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Str. 20, 53359 Rheinbach, Germany.
Institute of Organic Chemistry and Macromolecular Chemistry, Center of Excellence of Polysaccharide Research, Friedrich Schiller University of Jena, Humboldtstr. 10, 07743 Jena, Germany.
Int J Mol Sci. 2019 Jul 21;20(14):3565. doi: 10.3390/ijms20143565.
Healing of large bone defects requires implants or scaffolds that provide structural guidance for cell growth, differentiation, and vascularization. In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system. Hydroxyapatite (HA) was incorporated into the agarose gels in situ in various ratios by a simple procedure consisting of precipitation, cooling, washing, and drying. The resulting gels were characterized regarding composition, porosity, mechanical properties, and biocompatibility. A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers. Mechanical testing revealed elastic moduli of up to 2.8 MPa for lyophilized composites. MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds. Furthermore, scaffolds were loaded with model drug compounds for guided hMSC differentiation. Different release kinetic models were evaluated for adenosine 5'-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.
治疗大骨缺损需要植入物或支架，为细胞生长、分化和血管生成提供结构指导。在本工作中，开发了一种琼脂糖-羟基磷灰石复合支架，它不仅可以作为 3D 基质，还可以作为释放系统。通过简单的沉淀、冷却、洗涤和干燥过程，将羟基磷灰石（HA）以不同比例原位掺入琼脂糖凝胶中。对所得凝胶的组成、孔隙率、力学性能和生物相容性进行了表征。支架中鉴定出了纯相碳酸化 HA，其孔径可达数百微米。冻干复合材料的弹性模量高达 2.8 MPa。MTT 试验对 Lw35 人骨髓间充质干细胞（hMSC）和骨肉瘤 MG-63 细胞的检测证明了支架的生物相容性。此外，支架还负载了模型药物化合物，用于指导 hMSC 分化。对三磷酸腺苷（ATP）和苏拉明进行了不同的释放动力学模型评估，数据显示在四天内具有持续释放行为。
