Yager Eric J, Dean Hansi J, Fuller Deborah Heydenburg
Center for Immunology & Microbial Disease, Albany Medical College, Albany, NY 12208, USA.
Expert Rev Vaccines. 2009 Sep;8(9):1205-20. doi: 10.1586/erv.09.82.
Vaccine strategies capable of conferring broad protection against both seasonal and pandemic strains of influenza are urgently needed. DNA vaccines are an attractive choice owing to their capacity to induce robust humoral and cellular immune responses at low doses and because they can be developed and manufactured rapidly to more effectively meet the threat of an influenza epidemic or pandemic. Particle-mediated epidermal delivery (PMED), or the gene gun, is a DNA vaccine delivery technology shown to induce protective levels of antibody and T-cell responses in animals and humans against a wide variety of diseases, including influenza. This review focuses on current advances toward the development of an effective PMED DNA vaccine against influenza, including strategies to enhance vaccine immunogenicity, the potential for PMED-based DNA vaccines to improve protection in the vulnerable elderly population, and the prospects for a vaccine capable of providing cross-protection against both seasonal and pandemic strains of influenza.
迫切需要能够对季节性流感毒株和大流行性流感毒株都提供广泛保护的疫苗策略。DNA疫苗是一个有吸引力的选择，因为它们能够以低剂量诱导强大的体液免疫和细胞免疫反应，而且可以快速研发和生产，从而更有效地应对流感流行或大流行的威胁。颗粒介导的表皮递送（PMED），即基因枪，是一种DNA疫苗递送技术，已证明可在动物和人类中诱导针对包括流感在内的多种疾病的保护性抗体水平和T细胞反应。本综述重点关注开发有效的抗流感PMED DNA疫苗的当前进展，包括增强疫苗免疫原性的策略、基于PMED的DNA疫苗改善对脆弱老年人群保护的潜力，以及能够对季节性流感毒株和大流行性流感毒株都提供交叉保护的疫苗的前景。
