Bicho Diana, Queiroz João António, Tomaz Cândida Teixeira
CICS-UBI Health Science Research Center, Uiniversity of Beira Interior, P.O. Box: 6201-001, Covilha, Portugal.
Curr Gene Ther. 2015;15(6):541-9. doi: 10.2174/1566523215666150929111048.
Current influenza vaccines have long been used to fight flu infectious; however, recent advances highlight the importance of produce new alternatives. Even though traditional influenza vaccines are safe and usually effective, they need to be uploaded every year to anticipate circulating flu viruses. This limitation together with the use of embryonated chicken eggs as the substrate for vaccine production, is time-consuming and could involve potential biohazards in growth of new virus strains. Plasmid DNA produced by prokaryote microorganisms and encoding foreign proteins had emerged as a promising therapeutic tool. This technology allows the expression of a gene of interest by eukaryotic cells in order to induce protective immune responses against the pathogen of interest. In this review, we discuss the strategies to choose the best DNA vaccine to be applied in the treatment and prevention of influenza. Specifically, we give an update of influenza DNA vaccines developments, all involved techniques, their main characteristics, applicability and technical features to obtain the best option against influenza infections.
目前的流感疫苗长期以来一直用于对抗流感感染；然而，最近的进展凸显了开发新替代品的重要性。尽管传统流感疫苗安全且通常有效，但每年都需要更新以应对流行的流感病毒。这种局限性以及使用鸡胚作为疫苗生产的底物，既耗时又可能在新病毒株的生长过程中涉及潜在的生物危害。由原核微生物产生并编码外源蛋白的质粒DNA已成为一种有前景的治疗工具。这项技术允许真核细胞表达感兴趣的基因，以诱导针对感兴趣病原体的保护性免疫反应。在这篇综述中，我们讨论了选择最佳DNA疫苗用于治疗和预防流感的策略。具体而言，我们介绍了流感DNA疫苗的发展、所有涉及的技术、它们的主要特点、适用性以及获得对抗流感感染最佳选择的技术特征。
