白细胞弹性蛋白酶抑制剂：PARP-1的一种新型调节剂。

Leukocyte elastase inhibitor: a new regulator of PARP-1.

作者信息

Leprêtre Chloé, Sidoli Giuseppina, Scovassi A Ivana, Torriglia Alicia

机构信息

Université Pierre et Marie Curie-Paris 6, Paris, France.

出版信息

Ann N Y Acad Sci. 2009 Aug;1171:25-31. doi: 10.1111/j.1749-6632.2009.04701.x.

DOI:10.1111/j.1749-6632.2009.04701.x

PMID:19723034

Abstract

Poly(ADP-ribose) polymerase-1 (PARP-1) uses NAD(+) as a substrate to form ADP-ribose. During apoptosis, caspases cleave PARP-1 to avoid excessive NAD consumption. Because PARP-1 is a key regulator of the activity of DNases involved in caspase-dependent apoptosis, its cleavage is required to promote DNA degradation. To explore the situation in caspase-independent cell death, we investigated the effect of PARP-1 on the acid endonuclease leukocyte elastase inhibitor (LEI)-derived DNase II (L-DNase II). We found for the first time an association between PARP-1 and LEI/L-DNase II. Unexpectedly, we observed that LEI influenced the automodification of PARP-1.

摘要

聚（ADP - 核糖）聚合酶 -1（PARP -1）以烟酰胺腺嘌呤二核苷酸（NAD(+)）为底物形成ADP - 核糖。在细胞凋亡过程中，半胱天冬酶会切割PARP -1以避免NAD过度消耗。由于PARP -1是参与半胱天冬酶依赖性细胞凋亡的脱氧核糖核酸酶（DNase）活性的关键调节因子，其切割对于促进DNA降解是必需的。为了探究非半胱天冬酶依赖性细胞死亡的情况，我们研究了PARP -1对酸性核酸内切酶白细胞弹性蛋白酶抑制剂（LEI）衍生的DNase II（L - DNase II）的影响。我们首次发现了PARP -1与LEI/L - DNase II之间的关联。出乎意料的是，我们观察到LEI影响了PARP -1的自身修饰。