Jang Young Jin, Kang Nam Joo, Lee Ki Won, Lee Hyong Joo
Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Ann N Y Acad Sci. 2009 Aug;1171:170-5. doi: 10.1111/j.1749-6632.2009.04720.x.
There is accumulating evidence that a moderate consumption of red wine has health benefits, such as the inhibition of neurodegenerative diseases. Although this is generally attributed to resveratrol, the protective mechanisms and the active substance(s) remain unclear. We examined whether and how red wine extract (RWE) and red wine flavonols quercetin and myricetin inhibited 4-hydroxynonenal (HNE)-induced apoptosis of rat pheochromocytoma PC12 cells. RWE attenuated HNE-induced PC12 cell death in a dose-dependent manner. HNE induced cleavage of poly(ADP-ribose) polymerase, which is involved in DNA repair in the nucleus, and this was inhibited by RWE treatment. Treatment with RWE also inhibited HNE-induced nuclear condensation in PC12 cells. Data of 2',7'-dichlorofluorescin diacetate showed that RWE protected against apoptosis of PC12 cells by attenuating intracellular reactive oxygen species. The cytoprotective effects on HNE-induced cell death were stronger for quercetin and myricetin than for resveratrol. HNE-induced nuclear condensation was attenuated by quercetin and myricetin. These results suggest that the neuroprotective potential of red wine is attributable to flavonols rather than to resveratrol.
越来越多的证据表明，适量饮用红酒对健康有益，比如可抑制神经退行性疾病。尽管这通常归因于白藜芦醇，但其保护机制和活性物质仍不清楚。我们研究了红酒提取物（RWE）以及红酒中的黄酮醇槲皮素和杨梅素是否以及如何抑制4-羟基壬烯醛（HNE）诱导的大鼠嗜铬细胞瘤PC12细胞凋亡。RWE以剂量依赖的方式减轻了HNE诱导的PC12细胞死亡。HNE诱导了参与细胞核DNA修复的聚（ADP-核糖）聚合酶的裂解，而RWE处理可抑制这种裂解。RWE处理还抑制了HNE诱导的PC12细胞核浓缩。二氯荧光素二乙酸酯的数据表明，RWE通过减轻细胞内活性氧来保护PC12细胞免受凋亡。槲皮素和杨梅素对HNE诱导的细胞死亡的细胞保护作用比对白藜芦醇更强。槲皮素和杨梅素减轻了HNE诱导的核浓缩。这些结果表明，红酒的神经保护潜力归因于黄酮醇而非白藜芦醇。
