DNA Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Toxicol In Vitro. 2010 Sep;24(6):1592-8. doi: 10.1016/j.tiv.2010.06.008. Epub 2010 Jun 22.
The role of 4-hydroxynonenal (4-HNE), a byproduct of membrane lipid peroxidation has been suggested in neurodegeneration. However, the underlying mechanisms are poorly understood. The investigations were carried out to study the preventive potential of trans-resveratrol against 4-HNE induced damage in PC12 cells. Trans-resveratrol, a natural compound obtained from grape skin and found in red wine, is reported to have wide pharmacological window. Cells pretreated with trans-resveratrol (5, 10 and 25 microM) for 24 h were exposed to 4-HNE (25 microM) for 2 h. Pre-treatment of trans-resveratrol was found to be significantly effective in countering the cytotoxic responses of 4-HNE. Significant reduction in reactive oxygen species generation, restoration of intracellular glutathione, and lipid peroxidation levels suggest the improved antioxidant defense system in the cells pretreated with trans-resveratrol. Further, 4-HNE induced alterations in the protein expression of mitochondria-mediated apoptosis markers (Bax, Bcl-2 and Caspase-3) were significantly restored by pre-treatment of trans-resveratrol suggesting the protective potential of trans-resveratrol in PC12 cells against 4-HNE induced oxidative damage. Together these data show the prophylactic potential of trans-resveratrol in oxidative stress mediated apoptotic neurodegeneration.
4-羟壬烯醛（4-HNE）是膜脂质过氧化的副产物，其在神经退行性变中的作用已被提出。然而，其潜在的机制尚不清楚。本研究旨在研究反式白藜芦醇对 PC12 细胞中 4-HNE 诱导损伤的预防潜力。反式白藜芦醇是一种从葡萄皮中提取的天然化合物，存在于红酒中，据报道具有广泛的药理作用。细胞用反式白藜芦醇（5、10 和 25μM）预处理 24 h 后，用 4-HNE（25μM）处理 2 h。结果发现，反式白藜芦醇预处理对拮抗 4-HNE 的细胞毒性反应非常有效。活性氧生成的显著减少、细胞内谷胱甘肽的恢复以及脂质过氧化水平的降低表明，反式白藜芦醇预处理可改善细胞的抗氧化防御系统。此外，反式白藜芦醇预处理可显著恢复 4-HNE 诱导的线粒体介导的细胞凋亡标志物（Bax、Bcl-2 和 Caspase-3）的蛋白表达改变，表明反式白藜芦醇对 PC12 细胞中 4-HNE 诱导的氧化损伤具有保护作用。这些数据表明，反式白藜芦醇在氧化应激介导的凋亡性神经退行性变中具有预防潜力。
