Dasgupta Anindya, Peirce Susan K, Findley Harry W
Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.
Oncol Rep. 2009 Oct;22(4):831-5. doi: 10.3892/or_00000506.
Our previous studies have suggested that MycN may have a role in regulating livin expression in neuroblastoma. Here, we show that siRNA-mediated repression of MycN in neuroblastoma cells with both elevated MycN and livin expression resulted in significant downregulation of livin. Conversely, induction of MycN in neuroblastoma cells with low endogenous levels of MycN and livin protein upregulated livin expression. MycN directly associated with its regulatory motif (E-box) within the putative livin promoter. Based on these results, we hypothesize that MycN is required for livin expression and that livin may counteract the proapoptotic effects of MycN.
我们之前的研究表明，MycN可能在调节神经母细胞瘤中livin的表达方面发挥作用。在此，我们发现，在MycN和livin表达均升高的神经母细胞瘤细胞中，siRNA介导的MycN抑制导致livin显著下调。相反，在MycN和livin蛋白内源性水平较低的神经母细胞瘤细胞中诱导MycN可上调livin表达。MycN与其假定的livin启动子内的调控基序（E盒）直接相关。基于这些结果，我们推测MycN是livin表达所必需的，并且livin可能抵消MycN的促凋亡作用。
