Division of Allergy/Immunology and Infectious Diseases, University of Medicine and Dentistry of New Jersey (UMDNJ), New Jersey Medical School (NJMS), Newark, NJ 07101-1709, USA.
Pediatr Allergy Immunol. 2010 Jun;21(4 Pt 2):e747-55. doi: 10.1111/j.1399-3038.2009.00923.x. Epub 2009 Aug 27.
Toll like receptors (TLR) regulate innate immune responses sensing byproducts of intestinal microbiota. We examined responses to TLR agonists in children with inflammatory bowel disease (IBD). Peripheral blood mononuclear cells (PBMC) obtained from children with IBD [Crohn's disease (CD, n = 10), ulcerative colitis (UC, n = 10)], children with non-IgE-mediated food allergy (NFA, n = 20), and controls (n = 15) were tested for their production of proinflammatory and counter-regulatory cytokines with TLR agonists in comparison with their cytokine production against milk protein and candida. IBD patients were all in the inactive state. IBD PBMC produced more IL-6 with all the TLR agonists tested than controls. CD PBMC produced more counter-regulatory cytokines with TLR agonists, while UC PBMC produced more IL-1ss and IL-10 with TLR 7/8 agonist than controls. Cytokine production by NFA PBMC did not differ from controls. CD but not UC PBMC produced more IFN-gamma and IL-17 with candida. Aberrant responses to TLR agonists may be associated with increase in IFN-gamma/IL-17 production against candida in CD children.
Toll 样受体（TLR）通过感应肠道微生物群的副产物来调节先天免疫反应。我们研究了 TLR 激动剂在炎症性肠病（IBD）患儿中的反应。从 IBD 患儿（克罗恩病（CD，n=10），溃疡性结肠炎（UC，n=10））、非 IgE 介导的食物过敏（NFA，n=20）和对照（n=15）中获得外周血单核细胞（PBMC），并用 TLR 激动剂检测其促炎和抗炎细胞因子的产生情况，并与牛奶蛋白和念珠菌的细胞因子产生情况进行比较。所有 IBD 患者均处于非活动状态。与对照组相比，IBD PBMC 产生更多的 IL-6 与所有 TLR 激动剂测试。CD PBMC 用 TLR 激动剂产生更多的抗炎细胞因子，而 UC PBMC 用 TLR7/8 激动剂产生更多的 IL-1ss 和 IL-10 与对照组相比。NFA PBMC 的细胞因子产生与对照组无差异。CD 但不是 UC PBMC 与念珠菌一起产生更多的 IFN-γ和 IL-17。对 TLR 激动剂的异常反应可能与 CD 患儿针对念珠菌产生 IFN-γ/IL-17 增加有关。
