Yeh Hung-Yueh, Klesius Phillip H
United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, 990 Wire Road, Auburn, AL 36832-4352, United States.
Vet Immunol Immunopathol. 2010 Feb 15;133(2-4):302-8. doi: 10.1016/j.vetimm.2009.08.006. Epub 2009 Aug 13.
CD63, known as lysosome associated membrane protein 3 (LAMP-3), is a member of the tetraspanin integral membrane protein family. This protein plays many important roles in immuno-physiological functions. In this communication, we report the identification, characterization, and expression analysis of the channel catfish CD63 transcript. The complete nucleic acid sequence of channel catfish CD63 cDNA was comprised of 1159 nucleotides, including an open reading frame, which appears to encode a putative peptide of 237-amino-acid residues. Like other tetraspanin proteins, the channel catfish CD63 peptide can be divided into domains, including four transmembrane domains, three intracellular domains, and one of each small and large extracellular loops. The channel catfish CD63 peptide shares 52-55% identity among fish counterparts, but only 43-46% identity among mammalian counterparts. The characteristic Cys-Cys-Gly motif and four Cys residues in the large extracellular loop were conserved. The channel catfish CD63 transcript was detected by RT-PCR in spleen, anterior kidney, liver, intestine, skin and gill. This result provides important information for further elucidating CD63 functions in channel catfish.
CD63，也被称为溶酶体相关膜蛋白3（LAMP - 3），是四跨膜蛋白家族的成员。该蛋白在免疫生理功能中发挥着许多重要作用。在本通讯中，我们报告了斑点叉尾鮰CD63转录本的鉴定、特征分析及表达分析。斑点叉尾鮰CD63 cDNA的完整核酸序列由1159个核苷酸组成，包括一个开放阅读框，该开放阅读框似乎编码一个237个氨基酸残基的假定肽段。与其他四跨膜蛋白一样，斑点叉尾鮰CD63肽段可分为多个结构域，包括四个跨膜结构域、三个细胞内结构域以及一个小的和一个大的细胞外环。斑点叉尾鮰CD63肽段与鱼类对应物的同源性为52 - 55%，但与哺乳动物对应物的同源性仅为43 - 46%。大细胞外环中的特征性半胱氨酸 - 半胱氨酸 - 甘氨酸基序和四个半胱氨酸残基是保守的。通过RT - PCR在脾脏、前肾、肝脏、肠道、皮肤和鳃中检测到斑点叉尾鮰CD63转录本。该结果为进一步阐明CD63在斑点叉尾鮰中的功能提供了重要信息。
