Yeh Hung-Yueh, Klesius Phillip H
United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, Auburn, AL 36832-4352, USA.
Vet Immunol Immunopathol. 2010 Apr 15;134(3-4):289-95. doi: 10.1016/j.vetimm.2009.09.022. Epub 2009 Oct 2.
Chemokine receptor CXCR4, a member of the G protein-coupled receptor superfamily, binds selectively CXCL12. This protein plays many important roles in immunological as well as pathophysiological functions. In this study, we identified and characterized the channel catfish CXCR4 transcript. The full-length nucleic acid sequence of channel catfish CXCR4 cDNA comprised of 1994 nucleotides, including an open reading frame, which appears to encode a putative peptide of 357 amino acid residues with a calculated molecular mass of 40.1kDa. By comparison with the human counterpart, the channel catfish CXCR4 peptide can be divided into domains, including seven transmembrane domains, four cytoplasmic domains, and four extracellular domains. The CXCR4 transcript was detected in spleen, anterior kidney, liver, intestine, skin and gill of all catfish examined in this study. Because four CXCL of channel catfish have been identified, the result provides valuable information for further exploring the channel catfish chemokine signalling pathways and their roles in immune responses to infection.
趋化因子受体CXCR4是G蛋白偶联受体超家族的成员，它能选择性地结合CXCL12。该蛋白在免疫以及病理生理功能中发挥着许多重要作用。在本研究中，我们鉴定并表征了斑点叉尾鮰CXCR4转录本。斑点叉尾鮰CXCR4 cDNA的全长核酸序列由1994个核苷酸组成，包括一个开放阅读框，该开放阅读框似乎编码一个由357个氨基酸残基组成的推定肽段，计算分子量为40.1 kDa。通过与人类对应物比较，斑点叉尾鮰CXCR4肽段可分为多个结构域，包括七个跨膜结构域、四个胞质结构域和四个胞外结构域。在本研究中检测的所有斑点叉尾鮰的脾脏、前肾、肝脏、肠道、皮肤和鳃中均检测到CXCR4转录本。由于已鉴定出斑点叉尾鮰的四种CXCL，该结果为进一步探索斑点叉尾鮰趋化因子信号通路及其在感染免疫反应中的作用提供了有价值的信息。
