Armstrong Michael, Liu Andrew H, Harbeck Ronald, Reisdorph Rick, Rabinovitch Nathan, Reisdorph Nichole
National Jewish Health, Department of Immunology, 1400 Jackson Street K924, Denver, CO 80206, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Oct 1;877(27):3169-74. doi: 10.1016/j.jchromb.2009.08.011. Epub 2009 Aug 18.
A new analytical method suitable for high throughput measurements of LTE(4) in human urine is described. The methodology utilizes on-line enrichment and liquid chromatography/tandem mass spectrometry (LC/MS/MS). The novel LC/MS/MS method is rapid, linear from 5 to 500pg/ml in spiked urine samples of both healthy and asthmatic subjects and more accurate and precise than enzyme immunoassay (EIA) and previous LC/MS/MS methods. Results from sample integrity experiments and preliminary values of urinary LTE(4) from healthy adults and children are reported.
本文描述了一种适用于高通量检测人尿中LTE(4)的新分析方法。该方法采用在线富集和液相色谱/串联质谱法(LC/MS/MS)。这种新型的LC/MS/MS方法快速,在健康受试者和哮喘患者的加标尿样中,浓度在5至500pg/ml范围内呈线性,且比酶免疫分析(EIA)和以前的LC/MS/MS方法更准确、更精密。报告了样品完整性实验的结果以及健康成人和儿童尿中LTE(4)的初步值。