[通过二维凝胶电泳和质谱法鉴定人睾丸胚胎癌蛋白]
[Identification of human testicular embryonal carcinoma proteins by two-dimensional gel electrophoresis and mass spectrometry].
作者信息
Li Fei, Zou Ya-guang, Zhou Qi-zhao, Li Tie-qiu, Guo Wen-bin, Jing Xiao-wei, Mao Xiang-ming, Tan Wan-long, Zheng Shao-bin
机构信息
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Aug;29(8):1585-7.
OBJECTIVE
To separate and identify human testicular embryonal carcinoma proteomics using two-dimensional electrophoresis (2-DE) and mass spectrometry.
METHODS
Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis was used to separate the total proteins of the samples. After silver staining, PDQuest 7.30 image analysis software was applied to analyze the 2-DE images. Three of the proteins highly expressed in human testicular embryonal carcinoma were identified by matrix-assisted laser adsorption/ionization-time of flight-tandem mass spectrometry (MALDI-TOF-MS/MS).
RESULTS
2-DE effectively screened the differentially expressed proteins in the carcinoma tissues. Three proteins highly expressed in the carcinoma were successfully identified.
CONCLUSION
The proteins of human testicular embryonal carcinoma can be effectively separated and analyzed using 2-DE and mass spectrometry. Proteomic analysis offers a new means for further study of this carcinoma.
目的
采用二维电泳（2-DE）和质谱法分离并鉴定人睾丸胚胎癌蛋白质组学。
方法
使用固定化pH梯度二维聚丙烯酰胺凝胶电泳分离样品中的总蛋白质。银染后，应用PDQuest 7.30图像分析软件分析二维电泳图像。通过基质辅助激光吸附/电离飞行时间串联质谱（MALDI-TOF-MS/MS）鉴定了在人睾丸胚胎癌中高表达的三种蛋白质。
结果
二维电泳有效筛选出癌组织中差异表达的蛋白质。成功鉴定出在癌组织中高表达的三种蛋白质。
结论
采用二维电泳和质谱法可有效分离并分析人睾丸胚胎癌蛋白质。蛋白质组学分析为进一步研究该癌症提供了新手段。
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