Zuo Dan, Zhou Li-ning, Xie Feng, He Dan-dan, Mei Gui-ping
Department of Orthopedics, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Aug;29(8):1629-31.
To investigate the effects of co-exposure of LPS and heat on TNF-alpha expression in rat kidneys.
Male pathogen-free Wistar rats were randomly assigned in saline-injected normothermic control (C group), saline-injected heat exposure (H group), LPS-injected normothermic control (L group), and LPS-injected heat exposure (HL group). The rats in H and HL groups were exposed in a chamber at an ambient dry bulb temperature (Tdb) of 35.0-/+0.5 degrees, and those in C and L groups were exposed to a Tdb of 26-/+0.5 degrees. The rats in L and HL groups were given an intravenous injection of LPS (10 mg/kg) via the tail vein to induce endotoxemia, and equivalent normal saline was injected in C and H groups. TNF-alpha expression in the kidney was detected by immunohistochemical SABC method, and the renal damage was evaluated histologically at 120 min after the treatment.
Co-exposure of the rats with LPS and heat caused significantly enhanced TNF-alpha expression and histopathological damage in the kidneys.
LPS combined with heat exposure causes renal toxicity, while is closely associated with the expression of TNF-alpha in the kidneys.
研究脂多糖（LPS）与热共同暴露对大鼠肾脏肿瘤坏死因子-α（TNF-α）表达的影响。
将雄性无特定病原体Wistar大鼠随机分为生理盐水注射常温对照组（C组）、生理盐水注射热暴露组（H组）、LPS注射常温对照组（L组）和LPS注射热暴露组（HL组）。H组和HL组大鼠置于环境干球温度（Tdb）为35.0±0.5℃的舱内，C组和L组大鼠置于Tdb为26±0.5℃的环境中。L组和HL组大鼠经尾静脉注射LPS（10mg/kg）诱导内毒素血症，C组和H组注射等量生理盐水。采用免疫组织化学SABC法检测肾脏中TNF-α的表达，并在处理后120分钟对肾脏损伤进行组织学评估。
LPS与热共同暴露的大鼠肾脏中TNF-α表达显著增强，组织病理学损伤明显。
LPS与热暴露联合导致肾脏毒性，且与肾脏中TNF-α的表达密切相关。
