Zhang Jun, Sun Hong-mei, Bai Li-min, Xu Hong, Wu Hai-xia, Cui Long
Guangzhou University of Traditional Chinese Medicine, Guangzhou 510120, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Aug;29(8):1735-40.
To study the effect of the Chinese compound prescription Ginkgo biloba Pingchan Recipe (GBPR) on experimental Parkinson disease (PD) in mice and explore the possible mechanism.
Male C57/BL6J mice were divided into normal control, PD model and treatment groups. PD model was established by intraperitoneal injection with 1-methl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP) in the mice, and in the treatment group, GBPR was administered intragastrically after the injection. The mice were sacrificed 14 and 28 days later, and using in situ hybridization with Digoxin-labeled nNOS cDNA oligonucleotide probe, neuronal nitric oxide synthase (nNOS) mRNA was detected in the striatum and substantia nigra in the brain of mice.
nNOS mRNA expression was detected in the striatum and substantia nigra of the PD model mice, and GBPR treatment significantly reduced its expressions.
GBPR has obvious inhibitory effect against the neurotoxicity of NO probably by producing an anti-oxiditive effect through decreasing nNOS synthesis in the brain.
研究中药复方银杏平颤方（GBPR）对小鼠实验性帕金森病（PD）的影响并探讨其可能机制。
雄性C57/BL6J小鼠分为正常对照组、PD模型组和治疗组。通过腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）建立小鼠PD模型，治疗组在注射后灌胃给予GBPR。14天和28天后处死小鼠，采用地高辛标记的nNOS cDNA寡核苷酸探针原位杂交法，检测小鼠脑纹状体和黑质中神经元型一氧化氮合酶（nNOS）mRNA。
在PD模型小鼠的纹状体和黑质中检测到nNOS mRNA表达，GBPR治疗显著降低了其表达。
GBPR可能通过减少脑中nNOS合成产生抗氧化作用，对NO的神经毒性具有明显抑制作用。
