University of Tsukuba, Ibaraki, Japan.
J Biomater Appl. 2010 Jul;25(1):39-56. doi: 10.1177/0885328209342469. Epub 2009 Sep 2.
The effects of hardystonite (Ca(2)ZnSi(2)O(7), CSZn) and tricalcium phosphate (beta-TCP) on the proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) were compared by directly culturing MSCs on ceramic disks (contact mode) or separately culturing cells with ceramic disks (non-contact mode). In non-contact mode, the CSZn ceramic supported MSC proliferation more strongly than did the beta-TCP ceramic. However, in contact mode, the MSCs proliferated more quickly on the beta-TCP ceramic than they did on the CSZn ceramic. Alkaline phosphatase (ALP) staining and osteogenic gene expression analysis showed that the CSZn and beta-TCP ceramics had significant effects on the promotion of the osteogenic differentiation of MSCs in both non-contact and contact mode. Furthermore, in contact mode, the CSZn disk promoted the osteogenic differentiation of MSCs more strongly than did the beta-TCP disks. Even without the induction of dexamethasone and beta-glycerophosphate, CSZn stimulated the osteogenic differentiation of MSCs. These results suggest that CSZn ceramic would be a useful candidate material for bone regeneration and hard tissue engineering.
硬硅钙石（Ca(2)ZnSi(2)O(7)，CSZn）和磷酸三钙（β-TCP）对人骨髓间充质干细胞（MSCs）增殖和成骨分化的影响通过直接在陶瓷盘上培养 MSC（接触模式）或分别在陶瓷盘上培养细胞（非接触模式）进行了比较。在非接触模式下，CSZn 陶瓷比 β-TCP 陶瓷更能促进 MSC 增殖。然而，在接触模式下，MSCs 在 β-TCP 陶瓷上的增殖速度快于在 CSZn 陶瓷上的增殖速度。碱性磷酸酶（ALP）染色和成骨基因表达分析表明，CSZn 和 β-TCP 陶瓷在非接触和接触模式下均对 MSCs 成骨分化的促进具有显著作用。此外，在接触模式下，CSZn 盘比 β-TCP 盘更能促进 MSCs 的成骨分化。即使没有地塞米松和β-甘油磷酸的诱导，CSZn 也能刺激 MSCs 的成骨分化。这些结果表明，CSZn 陶瓷将是骨再生和硬组织工程的有用候选材料。
