Spinella S, Milon G, Hontebeyrie-Joskowicz M
Unité d'Immunoparasitologie, Institut Pasteur, Paris, France.
Eur J Immunol. 1990 May;20(5):1045-51. doi: 10.1002/eji.1830200515.
In our study we describe further characteristics of a CD4+ T cell line (G-05) isolated from lymph nodes of C3H/HeJ mice chronically infected with Trypanosoma cruzi. This T cell line secreted lymphokines such as interleukin (IL) 4 and IL 5 and could be defined as a TH2 type of helper T cells. By passive transfer into naive mice, the G-05 line was able to induce a polyclonal B cell activation in the spleen. This splenic B cell activation was quite similar to that seen in chronically T. cruzi-infected animals, where the isotypic pattern presents a large increase of IgG2a and IgG2b isotypes. Moreover, it was possible to reproduce this kind of polyclonal B cell activation in vivo, with the supernatant of G-05 T cells cultured in the presence of T. cruzi extract, accessory cells and exogenous IL 2. Analysis of this supernatant showed the presence of large amounts of IL 4, IL 5, IL 3 and IL 6 but not of interferon-gamma, and residual IL 2 activity was not significant. These results suggest that the G-05 T cells considered as TH2 cells on the basis of their lymphokine production are involved in the development of the in vivo polyclonal B cell activation in T. cruzi infection.
在我们的研究中，我们进一步描述了从慢性感染克氏锥虫的C3H/HeJ小鼠淋巴结中分离出的一种CD4 + T细胞系（G - 05）的特征。该T细胞系分泌白细胞介素（IL）4和IL 5等淋巴因子，可被定义为TH2型辅助性T细胞。通过被动转移至未感染的小鼠体内，G - 05细胞系能够诱导脾脏中的多克隆B细胞活化。这种脾脏B细胞活化与慢性克氏锥虫感染动物中所见的情况非常相似，在慢性感染动物中，同种型模式显示IgG2a和IgG2b同种型大幅增加。此外，用在克氏锥虫提取物、辅助细胞和外源性IL 2存在的情况下培养的G - 05 T细胞的上清液，有可能在体内重现这种多克隆B细胞活化。对该上清液的分析表明存在大量的IL 4、IL 5、IL 3和IL 6，但不存在干扰素 - γ，并且残余的IL 2活性不显著。这些结果表明，基于其淋巴因子产生而被视为TH2细胞的G - 05 T细胞参与了克氏锥虫感染中体内多克隆B细胞活化的发展。
