Park Joowon, Kim Jae Kyoung, Rheem Insoo, Kim Jongwan
Department of Laboratory Medicine, Dankook University Hospital, Cheonan, Korea.
Korean J Lab Med. 2009 Aug;29(4):307-13. doi: 10.3343/kjlm.2009.29.4.307.
Rapid identification of the causative agent among potential bacterial and viral pathogens is important for the management of acute respiratory disease. In this study, we evaluated the analytical performance and clinical usefulness of a recently-introduced multiplex PCR assay, Seeplex Pneumobacter detection kit (Seegene Inc., Korea) for the identification of respiratory bacterial pathogens.
One hundred and eighty one nasopharyngeal aspirates were collected from pediatric patients with respiratory symptoms and analysed by multiplex PCR for the detection of Streptococcus pneumoniae (S.P), Haemophilus influenzae (H.I), Mycoplasma pneumoniae (M.P), Chlamydophila pneumoniae (C.P), Bordetella pertussis (B.P) and Legionella pneumophila (L.P). A comparison of multiplex PCR with conventional culture for the isolation of S.P and H.I was performed on 112 specimens. The cross reactivity of multiplex PCR was also evaluated.
Of 181 cases, 81 cases were positive by multiplex PCR (44.8%): 52 cases for S.P (28.7%), 47 cases for H.I (26.0%), 9 cases for M.P (5.0%), 3 cases for B.P (1.7%) and 1 case for C.P (0.6%) including multiple infection cases. The agreement rates between multiplex PCR and culture for S.P and H.I were 92.9% (kappa index=0.84, P<0.001) and 91.1% (kappa index=0.75, P<0.001), respectively. There was no cross reactivity with common bacterial and viral pathogens.
Seeplex Pneumobacter detection kit could be a useful screening tool for the rapid detection of respiratory bacterial pathogens. Further studies with lower respiratory tract specimens would be needed for the clinical evaluation of S. pneumoniae and H. influenzae detected by multiplex PCR.
在潜在的细菌和病毒病原体中快速鉴定病原体对于急性呼吸道疾病的管理至关重要。在本研究中，我们评估了最近推出的多重PCR检测方法Seeplex Pneumobacter检测试剂盒（韩国Seegene公司）用于鉴定呼吸道细菌病原体的分析性能和临床实用性。
收集181例有呼吸道症状的儿科患者的鼻咽抽吸物，通过多重PCR检测肺炎链球菌（S.P）、流感嗜血杆菌（H.I）、肺炎支原体（M.P）、肺炎衣原体（C.P）、百日咳博德特氏菌（B.P）和嗜肺军团菌（L.P）。对112份标本进行多重PCR与传统培养法分离S.P和H.I的比较。同时评估多重PCR的交叉反应性。
181例病例中，多重PCR检测阳性81例（44.8%）：S.P 52例（28.7%），H.I 47例（26.0%），M.P 9例（5.0%），B.P 3例（1.7%），C.P 1例（0.6%），包括多重感染病例。多重PCR与培养法检测S.P和H.I的符合率分别为92.9%（kappa指数=0.84，P<0.001）和91.1%（kappa指数=0.75，P<0.001）。与常见细菌和病毒病原体无交叉反应。
Seeplex Pneumobacter检测试剂盒可能是快速检测呼吸道细菌病原体的有用筛查工具。对于多重PCR检测出的肺炎链球菌和流感嗜血杆菌，需要对下呼吸道标本进行进一步研究以进行临床评估。
