在感染肿瘤病毒的细胞中对病毒特异性糖蛋白合成进行非抗体依赖性检测。
Antibody-independent detection of virus-specific glycoprotein synthesis is oncornavirus-infected cells.
作者信息
England J M, Dietzschold B, Halpern M S
出版信息
J Virol. 1977 Sep;23(3):820-4. doi: 10.1128/JVI.23.3.820-824.1977.
Abstract
A method is described for detecting the synthesis of avian and murine oncornavirus-specific glycoproteins without the use of antibodies raised against viral structural proteins. As applied to cells infected with avian tumor virus, the method served to resolve pr90, the precursor of the major envelope glycoprotein. A virus-specific glycoprotein of about 85,000 daltons, which has several properties expected to a precursor to gp69/71, was detected in cells infected with murine leukemia virus.
摘要
本文描述了一种无需使用针对病毒结构蛋白产生的抗体来检测禽和鼠类肿瘤病毒特异性糖蛋白合成的方法。应用于感染禽肿瘤病毒的细胞时，该方法用于解析主要包膜糖蛋白的前体pr90。在感染鼠白血病病毒的细胞中检测到一种约85,000道尔顿的病毒特异性糖蛋白，它具有一些预期为gp69/71前体的特性。
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Biosynthesis of Rauscher leukemia viral proteins: presence of p30 and envelope p15 sequences in precursor polypeptides.劳斯氏白血病病毒蛋白的生物合成：前体多肽中存在p30和包膜p15序列。
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