Tung J S, Yoshiki T, Fleissner E
Cell. 1976 Dec;9(4 Pt 1):573-8. doi: 10.1016/0092-8674(76)90039-8.
A polypeptide of molecular weight approximately 75,000 daltons, p(75), was identified on the surface of AKR spontaneous leukemia cells by lactoperoxidase-catalyzed radio-iodination. This protein was shown by immunoprecipitation to have antigenic determinants of MuLV p30, p15, and p10, but not gp70, suggesting that p(75) represents a polyprotein composed of virion core components. As evidenced by studies on incorporation of radioactive glucosamine, p(75) is probably glycosylated. No p(75) was found on 2 month old AKR thymocytes, and only a small amount of p(75) was detectable of thymocytes from 4 month old animals. However, substantial quantities of p(75) could be found on thymocytes from 6 month old, yet still preleukemic mice.
通过乳过氧化物酶催化的放射性碘化，在AKR自发白血病细胞表面鉴定出一种分子量约为75,000道尔顿的多肽p(75)。免疫沉淀显示该蛋白具有莫氏白血病病毒（MuLV）p30、p15和p10的抗原决定簇，但不具有gp70的抗原决定簇，这表明p(75)代表一种由病毒体核心成分组成的多蛋白。对放射性葡糖胺掺入的研究表明，p(75)可能被糖基化。在2月龄的AKR胸腺细胞上未发现p(75)，在4月龄动物的胸腺细胞中仅可检测到少量p(75)。然而，在6月龄但仍处于白血病前期的小鼠的胸腺细胞上可发现大量p(75)。
