Department of Animal Physiology, Institute of Biology, M. Curie-Skłodowska University, 19 Akademicka Street, 20-033 Lublin, Poland.
Neurochem Res. 2010 Feb;35(2):262-72. doi: 10.1007/s11064-009-0050-5. Epub 2009 Aug 29.
Sublethal stress stimuli such as systemic endotoxin treatment can induce tolerance of the brain to subsequent ischemic stress, which results in a decreased infarct size. Based on this evidence, we hypothesized that lipopolysaccharide (LPS)-induced preconditioning could protect hippocampal neurons in epileptic rats. To test this hypothesis, the anticonvulsant effect of a low dose of LPS against seizures elicited by pilocarpine hydrochloride was measured. Using the pilocarpine model of temporal lobe epilepsy and LPS-preconditioning, we also investigated hippocampal pathology in the rat brain. Based on the behavioural observations conducted, it can be assumed that the preconditioning procedure used may decrease seizure excitability in epileptic rats. However, determination of the seizure excitability threshold needs to be elaborated. Qualitative and quantitative analyses of histological brain sections in the LPS-preconditioned rats showed markedly decreased intensity of neurodegenerative changes in the CA1, CA3 and DG hippocampal fields. The tendency was observed in all the periods of the pilocarpine model of epilepsy. We suggest that preconditioning with LPS may have neuroprotective effects in the CA1, CA3 and DG hippocampal sectors; however, it has no influence on the course of the seizures in rats in the pilocarpine model of epilepsy.
亚致死应激刺激，如全身内毒素处理，可以诱导大脑对随后的缺血性应激产生耐受，从而导致梗死面积减小。基于这一证据，我们假设脂多糖（LPS）诱导的预处理可以保护癫痫大鼠的海马神经元。为了验证这一假设，我们测量了低剂量 LPS 对盐酸匹鲁卡品诱发的癫痫发作的抗惊厥作用。使用颞叶癫痫的匹鲁卡品模型和 LPS 预处理，我们还研究了大鼠大脑中的海马病理学。根据进行的行为观察，可以假设使用的预处理程序可能会降低癫痫大鼠的癫痫发作兴奋性。然而，需要详细说明癫痫发作兴奋性阈值的测定。LPS 预处理大鼠的组织学脑切片的定性和定量分析显示，海马 CA1、CA3 和 DG 区神经退行性变化的强度明显降低。这种趋势在癫痫的匹鲁卡品模型的所有时期都观察到。我们认为，LPS 预处理可能对 CA1、CA3 和 DG 海马区具有神经保护作用；然而，它对匹鲁卡品模型癫痫大鼠的癫痫发作过程没有影响。
