Jaworska-Adamu Jadwiga, Dmowska Mirosława, Cybulska Regina, Krawczyk Aleksandra, Pawlikowska-Pawlęga Bożena
Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland.
Folia Histochem Cytobiol. 2011;49(2):219-24. doi: 10.5603/fhc.2011.0030.
The present paper is the first work to determine the effect of lipopolysaccharide (LPS) in the pilocarpine model of epilepsy on the morphology of rat hippocampal astrocytes in vivo. The study involved adult male Wistar rats, which 72 hours prior to administration of pilocarpine hydrochloride (PILO) were intraperitoneally (ip) preconditioned with LPS at a dose of 0.5 mg/kg b.w. The control animals were administered (ip) saline or LPS alone. The astrocytes in the control animals displayed characteristic stellate morphology. Examinations of the astrocytes were performed on days one, three and 21 of the pilocarpine model of epilepsy (i.e. in the acute, silent and chronic periods). The astrocytes of the CA1 and CA3 pyramidal layers of the hippocampus were observed and analyzed at the structural and ultrastructural levels. It was demonstrated that on days one and three, glial cells from both the nonpreconditioned and the LPS-preconditioned animals displayed similar reactive changes, manifesting themselves as swelling of cell bodies, glial processes, and astrocytosis. Moreover, reduction in cell organelles aggregated at one pole and the presence of vacuoles were observed. The most pronounced astrogliosis and cell swelling occurred on day 21. We conclude that LPS has no effect on the morphology of astrocytes in the pilocarpine model of epilepsy, unlike the results obtained by other authors in vitro.
本文是第一项在体内确定脂多糖（LPS）对匹罗卡品癫痫模型中大鼠海马星形胶质细胞形态影响的研究。该研究使用成年雄性Wistar大鼠，在腹腔注射盐酸匹罗卡品（PILO）前72小时，以0.5mg/kg体重的剂量对其进行腹腔内（ip）LPS预处理。对照动物腹腔注射生理盐水或单独注射LPS。对照动物中的星形胶质细胞呈现出典型的星形形态。在匹罗卡品癫痫模型的第1天、第3天和第21天（即急性期、静止期和慢性期）对星形胶质细胞进行检查。在结构和超微结构水平观察并分析海马CA1和CA3锥体层的星形胶质细胞。结果表明，在第1天和第3天，未预处理和LPS预处理动物的胶质细胞均表现出相似的反应性变化，表现为细胞体、胶质突起肿胀以及星形胶质细胞增生。此外，观察到细胞器在一极聚集减少以及存在空泡。最明显的星形胶质细胞增生和细胞肿胀发生在第21天。我们得出结论，与其他作者在体外获得的结果不同，LPS对匹罗卡品癫痫模型中的星形胶质细胞形态没有影响。
