Jiangsu Key Lab for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 210097 Nanjing, People's Republic of China.
Mol Biol Rep. 2010 Jul;37(6):2587-92. doi: 10.1007/s11033-009-9778-x. Epub 2009 Sep 2.
EJO1, a novel gene presumably involved in the ovary development of the Chinese mitten crab (Eriocheir japonica sinensis), was identified and characterized by suppression subtractive hybridization and cDNA macroarray analysis. EJO1 expression was 2.6-fold higher at stage III than at stage II during the ovary development of the mitten crab. EJO1 is 876 bp in length containing a 759 bp open reading frame which encodes a 252-amino-acid protein. Homology analysis showed that no sequence significantly matching EJO1 was found in SwissProt, so it was deduced as a novel gene (GenBank accession number: AY185917). The EJO1 protein is probably a secretion protein with a signal peptide of 17 amino acids. The pI/Mw deduced from the amino acid sequence was 6.18/28.18 kDa. Expression profile showed that EJO1 mRNA is highly expressed in the heart, intestine, and ovary of the crab, while there is little or no expression in muscle and hepatopancreas. The differential expression of EJO1 at the different developmental stages of the ovary was further confirmed by Northern blot analysis. In conclusion, EJO1 is a novel gene differentially expressed in the ovary of the Chinese mitten crab, which may play an important role in the ovary development.
EJO1 是一种新基因，可能参与中华绒螯蟹（Eriocheir japonica sinensis）的卵巢发育。本研究通过抑制性消减杂交和 cDNA 微阵列分析对其进行了鉴定和特征分析。在中华绒螯蟹卵巢发育过程中，EJO1 在 III 期的表达量是 II 期的 2.6 倍。EJO1 长 876bp，包含一个 759bp 的开放阅读框，编码一个 252 个氨基酸的蛋白质。同源性分析表明，在 SwissProt 中没有发现与 EJO1 序列显著匹配的序列，因此推断其为一个新基因（GenBank 登录号：AY185917）。EJO1 蛋白可能是一种分泌蛋白，具有 17 个氨基酸的信号肽。根据氨基酸序列推断的 pI/Mw 为 6.18/28.18kDa。表达谱显示，EJO1mRNA 在蟹的心、肠和卵巢中高度表达，而在肌肉和肝胰腺中表达很少或没有。Northern blot 分析进一步证实了 EJO1 在卵巢不同发育阶段的差异表达。总之，EJO1 是中华绒螯蟹卵巢中差异表达的新基因，可能在卵巢发育中发挥重要作用。
