Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Normal University, Yancheng 224051, Jiangsu, China.
Mol Biol Rep. 2011 Nov;38(8):4935-40. doi: 10.1007/s11033-010-0636-7. Epub 2010 Dec 14.
Mitochondrial control region was called "A + T-rich" region in invertebrate. In the study, the general organization of control region in mitten crab was divided into two major domains: high variable segment and conserved segment. Four conserved blocks (CSB1, CSB2, CSB3 and CSB4) and two tandem repeat sequences (RT1 and RT2) were defined in control region. There were 116 polymorphic sites and 84 parsimony information sites in 571 aligned sites of the high variable segment adjacent "tRNA-Gln", in which 58 stable variable sites were defined between E. j. sinensis and E. j. hepuensis. Conserved domain contained more than two similar repeat units, and length polymorphism of control region was due to the number difference between the two repeat units (RT1 and RT2). And length polymorphism was a common phenomenon for tandem repeat in control region in the study. Furthermore, a novel result showed the core nucleotide of RT2 in control region tandem repeat was C in E. j. hepuensis, but G in E. j. sinensis. It might be a rapid and cost-effective measure of seedlings differentiation in aquaculture.
线粒体控制区在无脊椎动物中被称为“富含 A+T”区。在本研究中，中华绒螯蟹的控制区总体结构被划分为两个主要区域：高变区和保守区。控制区中定义了四个保守区（CSB1、CSB2、CSB3 和 CSB4）和两个串联重复序列（RT1 和 RT2）。在相邻“tRNA-Gln”的高变区 571 个对齐位点中，有 116 个多态性位点和 84 个简约信息位点，其中中华绒螯蟹和日本绒螯蟹之间定义了 58 个稳定可变位点。保守区包含两个以上相似的重复单元，控制区的长度多态性是由于两个重复单元（RT1 和 RT2）之间的数量差异所致。而且，在本研究中，串联重复的控制区长度多态性是一个常见现象。此外，一个新的结果表明，控制区串联重复 RT2 的核心核苷酸在日本绒螯蟹中是 C，而在中国绒螯蟹中是 G。这可能是水产养殖中苗种分化的一种快速、经济有效的衡量标准。
