Haustein U F, Schliebs R, Schaller J
Department of Dermatology, Karl Marx University, Leipzig, G.D.R.
Acta Derm Venereol. 1990;70(3):208-11.
Muscarinic acetylcholine receptors were studied in slices of unaffected skin and in lymphocyte membranes from 3 patients with cholinergic urticaria and 5 healthy subjects using receptor autoradiography and the radioligand [3H]quinuclidinyl benzilate binding technique. Autoradiography revealed that muscarinic acetylcholine receptors were located in regions with sweat glands. The distribution of receptors did not differ qualitatively between patients and controls. However, the maximum number of muscarinic acetylcholine receptors was reduced in skin from patients with cholinergic urticaria, by 44% as compared with the controls, whereas the binding affinity was not affected. Muscarinic cholinergic binding in lymphocyte membrane fractions obtained from patients with cholinergic urticaria did not differ statistically from that of healthy control persons.
采用受体放射自显影术和放射性配体[3H]喹核醇基苯甲酸酯结合技术，对3例胆碱能性荨麻疹患者和5名健康受试者的未受影响皮肤切片及淋巴细胞膜中的毒蕈碱型乙酰胆碱受体进行了研究。放射自显影显示，毒蕈碱型乙酰胆碱受体位于有汗腺的区域。患者和对照组之间受体的分布在性质上没有差异。然而，胆碱能性荨麻疹患者皮肤中毒蕈碱型乙酰胆碱受体的最大数量减少，与对照组相比减少了44%，而结合亲和力未受影响。从胆碱能性荨麻疹患者获得的淋巴细胞膜组分中的毒蕈碱型胆碱能结合与健康对照者相比，在统计学上没有差异。
