Schwartz R D
Life Sci. 1986 Jun 9;38(23):2111-9. doi: 10.1016/0024-3205(86)90210-9.
The relative distribution of muscarinic and nicotinic cholinergic receptors labeled with [3H]acetylcholine was determined using autoradiography. [3H]Acetylcholine binding to high affinity muscarinic receptors was similar to what has been described for an M-2 distribution: highest levels of binding occurred in the pontine and brainstem nuclei, anterior pretectal area and anteroventral thalamic nucleus, while lower levels occurred in the caudate-putamen, accumbens nucleus and primary olfactory cortex. Nicotinic receptors were labeled with [3H]acetylcholine to the greatest extent in the interpeduncular nucleus, several thalamic nuclei, medial habenula, presubiculum and superior colliculus, and to the least extent in the hippocampus and inferior colliculus. By using autoradiography to localize cholinergic binding sites throughout the brain it was observed that the distributions of high affinity muscarinic and nicotinic sites labeled with the endogenous ligand, [3H]acetylcholine are different from each other and are different from distributions of muscarinic and nicotinic sites labeled with muscarinic and nicotinic antagonists.
使用放射自显影法测定了用[3H]乙酰胆碱标记的毒蕈碱型和烟碱型胆碱能受体的相对分布。[3H]乙酰胆碱与高亲和力毒蕈碱型受体的结合情况与已描述的M-2分布相似：结合水平最高的部位是脑桥和脑干核、顶盖前区和丘脑前腹侧核，而尾状核-壳核、伏隔核和初级嗅觉皮层的结合水平较低。烟碱型受体在脚间核、几个丘脑核、内侧缰核、前扣带回和上丘中被[3H]乙酰胆碱标记的程度最大，而在海马和下丘中被标记的程度最小。通过使用放射自显影法在全脑定位胆碱能结合位点，观察到用内源性配体[3H]乙酰胆碱标记的高亲和力毒蕈碱型和烟碱型位点的分布彼此不同，且与用毒蕈碱型和烟碱型拮抗剂标记的毒蕈碱型和烟碱型位点的分布不同。
