Yang Renliang, Pasunooti Kalyan Kumar, Li Fupeng, Liu Xue-Wei, Liu Chuan-Fa
Division of Chemical Biology and Biotechnology, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
J Am Chem Soc. 2009 Sep 30;131(38):13592-3. doi: 10.1021/ja905491p.
A thiol group introduced on the gamma-carbon of lysine mediates robust native chemical ligation at both the alpha- and epsilon-amines in two consecutive steps. Desulfurization then affords the final product, in which the lysine residue at the ligation site has an isopeptide bond on its side chain. The method is useful for the synthesis of proteins containing special post-translational modifications on lysine.
在赖氨酸的γ-碳上引入的硫醇基团在两个连续步骤中介导了α-胺和ε-胺处的稳健的天然化学连接。然后脱硫得到最终产物，其中连接位点处的赖氨酸残基在其侧链上具有异肽键。该方法对于合成在赖氨酸上具有特殊翻译后修饰的蛋白质很有用。
