Seville Lorna A, Patterson Andrea J, Scott Karen P, Mullany Peter, Quail Michael A, Parkhill Julian, Ready Derren, Wilson Michael, Spratt David, Roberts Adam P
Division of Microbial Diseases, UCL Eastman Dental Institute, London, United Kingdom.
Microb Drug Resist. 2009 Sep;15(3):159-66. doi: 10.1089/mdr.2009.0916.
We have analyzed the total metagenomic DNA from both human oral and fecal samples derived from healthy volunteers from six European countries to determine the molecular basis for tetracycline and erythromycin resistance. We have determined that tet(M) and tet(W) are the most prevalent tetracycline resistance genes assayed for in the oral and fecal metagenomes, respectively. Additionally, tet(Q), tet(O), and tet(O/32/O) have been shown to be common. We have also shown that erm(B), erm(V), and erm(E) are common erythromycin resistance genes present in these environments. Further, we have demonstrated the ubiquitous presence of the Tn916 integrase in the oral metagenomes and the Tn4451 and Tn1549 integrase genes within the fecal metagenomes.
我们分析了来自六个欧洲国家健康志愿者的人类口腔和粪便样本中的总宏基因组DNA，以确定四环素和红霉素耐药性的分子基础。我们已经确定tet(M)和tet(W)分别是在口腔和粪便宏基因组中检测到的最普遍的四环素耐药基因。此外，tet(Q)、tet(O)和tet(O/32/O)也已被证明很常见。我们还表明，erm(B)、erm(V)和erm(E)是这些环境中常见的红霉素耐药基因。此外，我们已经证明Tn916整合酶在口腔宏基因组中普遍存在，而Tn4451和Tn1549整合酶基因在粪便宏基因组中存在。
