Cox J A, Felder C C, Henneberry R C
Laboratory of Molecular Biology, NINDS, National Institute of Health, Bethesda, Maryland 20892.
Neuron. 1990 Jun;4(6):941-7. doi: 10.1016/0896-6273(90)90147-8.
Using neurotoxicity and inositol phosphate release as criteria for receptor expression, we report the differential expression of excitatory amino acid receptor subtypes in cerebellar granule cells grown in serum-free media containing either high (25 mM) or low (5 mM) KCl. NMDA receptors are expressed in neurons grown in high, but not low, KCl. In contrast, ionotropic quisqualate receptors are expressed in neurons grown in low KCl, but not in those grown in high KCl. Addition of NMDA to cultures containing low KCl appears to mimic high KCl conditions: NMDA receptors are expressed, but ionotropic quisqualate receptors are not. Glutamate and kainate are toxic to cells grown in either condition.
以神经毒性和肌醇磷酸释放作为受体表达的标准，我们报告了在含有高（25 mM）或低（5 mM）氯化钾的无血清培养基中生长的小脑颗粒细胞中兴奋性氨基酸受体亚型的差异表达。NMDA受体在高氯化钾而非低氯化钾条件下生长的神经元中表达。相反，离子型quisqualate受体在低氯化钾条件下生长的神经元中表达，而在高氯化钾条件下生长的神经元中不表达。向含有低氯化钾的培养物中添加NMDA似乎模拟了高氯化钾条件：NMDA受体表达，但离子型quisqualate受体不表达。谷氨酸和海藻酸对在任何一种条件下生长的细胞均有毒性。
