Rapp J P, Dene H
Department of Medicine, Medical College of Ohio, Toledo 43699.
J Hypertens. 1990 May;8(5):457-62. doi: 10.1097/00004872-199005000-00010.
A previously described Pst I restriction fragment length polymorphism in Dahl rats at the Na+, K(+)-adenosine 5'-triphosphatase (ATPase) alpha 1 isoform locus was tested for cosegregation with blood pressure in segregating populations. Inbred Dahl salt-sensitive (S) and inbred Dahl salt-resistant (R) rats were crossed to produce F1 rats, and three segregating populations, F1 x R, F2 and F1 x S, were produced and raised on a high-salt diet for variable periods to attain the maximal blood pressure response in each population. In no segregating population was there a significant difference in blood pressure among genotypes at the Na+, K(+)-ATPase alpha 1 locus. Power calculations suggest that single-allele dosage effects of less than 7.5 mmHg were unlikely to be detected. It is concluded that either the S and R alleles at the Na+, K(+)-ATPase alpha 1 locus do not influence blood pressure under the conditions of these experiments (high dietary salt, Dahl rat genetic background) or, if they do, their effect is small.
在分离群体中，对先前描述的达尔大鼠在钠钾腺苷三磷酸酶（ATP酶）α1同工型基因座处的Pst I限制性片段长度多态性与血压的共分离情况进行了检测。将近交系达尔盐敏感（S）大鼠和近交系达尔盐抵抗（R）大鼠杂交产生F1大鼠，然后产生三个分离群体，即F1×R、F2和F1×S，并在高盐饮食下饲养不同时间，以在每个群体中获得最大血压反应。在任何分离群体中，钠钾ATP酶α1基因座处的基因型之间血压均无显著差异。功效计算表明，不太可能检测到小于7.5 mmHg的单等位基因剂量效应。结论是，在这些实验条件下（高盐饮食、达尔大鼠遗传背景），钠钾ATP酶α1基因座处的S和R等位基因要么不影响血压，要么即使有影响，其作用也很小。
