Morzaria S P, Spooner P R, Bishop R P, Musoke A J, Young J R
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Mol Biochem Parasitol. 1990 May;40(2):203-11. doi: 10.1016/0166-6851(90)90042-k.
DNAs of Theileria parva parva, T. p. lawrencei, T. p. bovis and Theileria mutans stocks, from Kenya, Uganda, Zanzibar and Zimbabwe were digested with either SfiI or NotI and analysed using contour-clamped homogeneous electric field (CHEF) and field-inversion gel electrophoresis (FIGE). The SfiI-digested T. parva genomic DNA resolved into approximately 30 fragments while the NotI digestion produced between 4-7 bands. The summation of the sizes of SfiI fragments gave an estimate of 9-10 X 10(6) base pairs for the size of the T. parva genome. Heterogeneity within T. p. parva Muguga, Pemba/Mnarani and Mariakani stocks was detected. All the T. parva stocks analysed showed SfiI and NotI restriction fragment length polymorphisms (RFLP). Hybridisation of 5 SfiI-digested T. parva DNAs with a Plasmodium berghei telomeric repeat probe suggested that most of the polymorphisms and heterogeneity occurred in the telomeric or sub-telomeric regions of the genome. The recognition by the Plasmodium telomeric probe of 7-8 strongly hybridising SfiI bands indicates that the T. parva genome may possess at least 4 chromosomes. The T. mutans genome was cut frequently with the above enzymes resulting in large numbers of fragments predominantly below 50 kb, thus suggesting either a much higher G + C content than T. parva or the presence of highly reiterated G + C-rich regions.
来自肯尼亚、乌干达、桑给巴尔和津巴布韦的微小泰勒虫、劳伦斯泰勒虫、牛泰勒虫和突变泰勒虫毒株的DNA，用SfiI或NotI进行消化，并用轮廓夹恒定电场（CHEF）和场反转凝胶电泳（FIGE）进行分析。经SfiI消化的微小泰勒虫基因组DNA可解析为约30个片段，而NotI消化产生4至7条带。SfiI片段大小的总和估计微小泰勒虫基因组大小为9 - 10×10⁶碱基对。检测到微小泰勒虫穆古加、奔巴/姆纳拉尼和马里卡尼毒株内的异质性。所有分析的微小泰勒虫毒株均显示出SfiI和NotI限制性片段长度多态性（RFLP）。用伯氏疟原虫端粒重复探针与5个经SfiI消化的微小泰勒虫DNA杂交表明，大多数多态性和异质性发生在基因组的端粒或亚端粒区域。伯氏疟原虫端粒探针识别7 - 8条强杂交的SfiI带，表明微小泰勒虫基因组可能至少拥有4条染色体。突变泰勒虫基因组被上述酶频繁切割，产生大量主要小于50 kb的片段，因此表明其G + C含量比微小泰勒虫高得多，或者存在高度重复的富含G + C的区域。
