在高浓度甲酰胺条件下RNA:DNA和DNA:DNA双链体的形成速率及热稳定性
Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.
作者信息
Casey J, Davidson N
出版信息
Nucleic Acids Res. 1977;4(5):1539-52. doi: 10.1093/nar/4.5.1539.
Abstract
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.
摘要
在含有高浓度甲酰胺的水性电解质溶液中，RNA:DNA杂交体的热稳定性显著高于DNA:DNA双链体。已在这些溶剂中测量了形成DNA:DNA双链体和DNA:RNA杂交体的缔合速率。存在一个温度范围，其中DNA:DNA的速率可忽略不计，而RNA:DNA的速率接近最佳值。
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