空气暴露、负压与溶血之间的关系。
The relationships between air exposure, negative pressure, and hemolysis.
作者信息
Pohlmann Joshua R, Toomasian John M, Hampton Claire E, Cook Keith E, Annich Gail M, Bartlett Robert H
机构信息
Department of Surgery, Division of Critical Care, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
出版信息
ASAIO J. 2009 Sep-Oct;55(5):469-73. doi: 10.1097/MAT.0b013e3181b28a5a.
The purpose of this study was to describe the hemolytic effects of both negative pressure and an air-blood interface independently and in combination in an in vitro static blood model. Samples of fresh ovine or human blood (5 ml) were subjected to a bubbling air interface (0-100 ml/min) or negative pressure (0-600 mm Hg) separately, or in combination, for controlled periods of time and analyzed for hemolysis. Neither negative pressure nor an air interface alone increased hemolysis. However, when air and negative pressure were combined, hemolysis increased as a function of negative pressure, the air interface, and time. Moreover, when blood samples were exposed to air before initiating the test, hemolysis was four to five times greater than samples not preexposed to air. When these experiments were repeated using freshly drawn human blood, the same phenomena were observed, but the hemolysis was significantly higher than that observed in sheep blood. In this model, hemolysis is caused by combined air and negative pressure and is unrelated to either factor alone.
本研究的目的是在体外静态血液模型中，分别描述负压和气血界面单独作用以及联合作用时的溶血效应。将新鲜的羊血或人血样本（5毫升）分别单独或联合暴露于鼓泡式空气界面（0 - 100毫升/分钟）或负压（0 - 600毫米汞柱）下，持续一定时间，然后分析溶血情况。单独的负压或空气界面均未增加溶血。然而，当空气和负压联合作用时，溶血随着负压、空气界面及时间的变化而增加。此外，在开始测试前将血样暴露于空气中时，溶血程度比未预先暴露于空气的样本高四至五倍。当使用新抽取的人血重复这些实验时，观察到相同的现象，但溶血程度显著高于在羊血中观察到的情况。在该模型中，溶血是由空气和负压共同作用引起的，与单一因素无关。
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