苯丙氨酸作为牛肾上腺嗜铬细胞中酪氨酸羟化酶的底物。
Phenylalanine as substrate for tyrosine hydroxylase in bovine adrenal chromaffin cells.
作者信息
Fukami M H, Haavik J, Flatmark T
机构信息
Department of Biochemistry, University of Bergen, Norway.
出版信息
Biochem J. 1990 Jun 1;268(2):525-8. doi: 10.1042/bj2680525.
Abstract
Incubation of bovine chromaffin cells with L-[14C]phenylalanine resulted in label accumulation in catecholamines at about 30% of the rate seen with L-tyrosine as precursor. Studies with purified tyrosine hydroxylase (EC 1.14.16.2) showed that the enzyme catalysed the hydroxylation of L-phenylalanine first to L-p-tyrosine and then to 3,4-dihydroxyphenylalanine (DOPA). No evidence for a significant involvement of an L-m-tyrosine intermediate in DOPA formation was found.
摘要
用L-[14C]苯丙氨酸孵育牛嗜铬细胞，导致儿茶酚胺中的标记积累，其速率约为以L-酪氨酸作为前体时所观察到速率的30%。对纯化的酪氨酸羟化酶（EC 1.14.16.2）的研究表明，该酶首先催化L-苯丙氨酸羟基化为L-p-酪氨酸，然后再羟基化为3,4-二羟基苯丙氨酸（多巴）。未发现L-m-酪氨酸中间体在多巴形成过程中起显著作用的证据。
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本文引用的文献
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