Energy and Environmental Engineering Division, National Institute of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea.
Biotechnol Lett. 2010 Jan;32(1):79-85. doi: 10.1007/s10529-009-0118-y.
Propionate was used as fuel to enrich an electrochemically-active microbial consortium in a microbial fuel cell, and the bacterial consortium was analyzed by culture-independent methods including denaturing gradient gel electrophoresis (DGGE) of the 16S rDNA, and by fluorescent in situ hybridization (FISH). MFCs fed with propionate produced a current of 4.88 +/- 0.1 mA stably on 100 mg propionate/l as COD within 3 weeks of the enrichment. When the MFCs were fed with H2-saturated fuel containing propionate, the current dropped to 3.82 +/- 0.07 mA. The maximum current generated was up to 8.8 mA when MFCs were fed with 200 mg propionate/l as COD. The DGGE of 16S rDNA showed that propionate- enriched MFCs have a different bacterial population from that enriched with acetate and from the inoculum used for enrichment. The major member (42%) of the consortium was an unidentified bacterium followed by c, b, and d-proteobacteria.
丙酸被用作燃料，在微生物燃料电池中富集电化学活性微生物群落，并用非培养方法（包括 16S rDNA 的变性梯度凝胶电泳（DGGE）和荧光原位杂交（FISH））分析细菌群落。在 3 周的富集期内，以丙酸作为 COD 的 100mg/L 丙酸进料的 MFC 可稳定产生 4.88±0.1mA 的电流。当 MFC 进料含有丙酸的 H2-饱和燃料时，电流下降至 3.82±0.07mA。当 MFC 以 200mg/L 丙酸作为 COD 进料时，最大电流高达 8.8mA。16S rDNA 的 DGGE 表明，丙酸富集的 MFC 具有与乙酸富集的 MFC 和富集用接种物不同的细菌种群。群落的主要成员（42%）是一种未鉴定的细菌，其次是 c、b 和 d-变形菌。
