利用16S rDNA序列分析贫营养微生物燃料电池中的微生物多样性。
Analysis of microbial diversity in oligotrophic microbial fuel cells using 16S rDNA sequences.
作者信息
Phung Nguyet Thu, Lee Jiyoung, Kang Kui Hyun, Chang In Seop, Gadd Geoffrey Michael, Kim Byung Hong
机构信息
Water Environment and Remediation Research Center, Korea Institute of Science and Technology, 39-1, Hawolgok dong, Sungbuk gu, Seoul 136-791, Republic of Korea.
出版信息
FEMS Microbiol Lett. 2004 Apr 1;233(1):77-82. doi: 10.1016/j.femsle.2004.01.041.
Molecular ecological techniques were applied to analyze the bacterial diversity of two oligotrophic microbial fuel cells (MFCs) enriched using river water or artificial wastewater (AWW) as fuel. Denaturing gradient gel electrophoresis (DGGE) of the PCR amplified 16S rDNA showed that different microbial communities were present in the two MFCs and these were different from the river sediment used to initiate the enrichment. Nearly complete 16S rDNA was amplified and sequenced. Over 80% of the clones were Proteobacteria. Betaproteobacteria were the dominant clones (46.2%) in MFCs fed with river water, and about 64.4% of the clones in MFCs fed with AWW were Alphaproteobacteria. Actinobacteria were found only in the MFC fed with AWW, and Deltaproteobacteria, Acidobacteria, Chloroflexi and Verrucomicrobia in the MFC fed with river water. Many clones were related to uncultured bacteria, some with homology less than 95%, indicating that many novel bacteria were enriched in the oligotrophic MFCs.
应用分子生态学技术分析了两个以河水或人工废水（AWW）为燃料富集培养的贫营养型微生物燃料电池（MFC）的细菌多样性。对PCR扩增的16S rDNA进行变性梯度凝胶电泳（DGGE）分析表明，两个MFC中存在不同的微生物群落，且这些群落与用于启动富集培养的河底沉积物中的微生物群落不同。扩增并测序了近乎完整的16S rDNA。超过80%的克隆属于变形菌门。在以河水为燃料的MFC中，β-变形菌是优势克隆（46.2%），而在以AWW为燃料的MFC中，约64.4%的克隆属于α-变形菌。放线菌仅在以AWW为燃料的MFC中被发现，而δ-变形菌、酸杆菌门、绿弯菌门和疣微菌门则在以河水为燃料的MFC中被发现。许多克隆与未培养细菌相关，有些克隆的同源性低于95%，这表明在贫营养型MFC中富集了许多新细菌。
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