Abarrategui Iratxe, Krangel Michael S
Centre for Epigenetics, Biotech Research and Innovation Centre, Copenhagen, Denmark.
Adv Exp Med Biol. 2009;650:93-102. doi: 10.1007/978-1-4419-0296-2_8.
The developmental control of V(D)J recombination is imposed at the level of chromatin accessibility of recombination signal sequences (RSSs) to the recombinase machinery. Cis-acting transcriptional regulatory elements such as promoters and enhancers play a central role in the control of accessibility in vivo. However, the molecular mechanisms by which these elements influence accessibility are still under investigation. Although accessibility for V(D)J recombination is usually accompanied by germline transcription at antigen receptor loci, the functional significance of this transcription in directing RSS accessibility has been elusive. In this chapter, we review past studies outlining the complex relationship between V(D)J recombination and transcription as well as our current understanding on how chromatin structure is regulated during gene expression. We then summarize recent work that directly addresses the functional role of transcription in V(D)J recombination.
V(D)J 重组的发育控制是在重组信号序列（RSSs）对重组酶机制的染色质可及性水平上施加的。顺式作用转录调控元件，如启动子和增强子，在体内可及性控制中起着核心作用。然而，这些元件影响可及性的分子机制仍在研究中。虽然 V(D)J 重组的可及性通常伴随着抗原受体基因座的种系转录，但这种转录在指导 RSS 可及性方面的功能意义一直难以捉摸。在本章中，我们回顾了过去的研究，概述了 V(D)J 重组与转录之间的复杂关系，以及我们目前对基因表达过程中染色质结构如何调控的理解。然后，我们总结了最近直接探讨转录在 V(D)J 重组中的功能作用的工作。
