Johnson R M, Lancki D W, Fitch F W, Spear P G
Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.
J Immunol. 1990 Jul 15;145(2):702-10.
Several previous reports have described the surprising inability to detect murine CTL specific for glycoprotein D (gD), one of the important protective immunogens of HSV. Using slight variations of published procedures, we were able to show that the immune response to HSV in infected mice includes the generation of CTL specific for gD. C3H/OuJ (H-2k) mice were infected by injection in the hind footpads with purified HSV-1. Lymphocytes from draining lymph nodes were then isolated and shown to proliferate in response to, and to kill, transformed fibroblasts (H-2k) expressing HSV-1 gD. Two gD-specific T cell clones were isolated. One clone, designated CGD1, was shwon to be CD8+. This clone recognizes HSV-1 gD, but not HSV-2 gD, in the context of class I MHC molecules and kills the appropriate MHC-matched fibroblasts expressing HSV-1 gD. Unusual features of this cytolytic clone include augmentation by IL-4 of proliferative responses to Ag, inhibition of its lytic activity by a mAb specific for Thy-1 and recognition of infected fibroblasts in preference to infected lymphoblasts. The other clone, designated CGD3, was shown to be CD4+. This clone recognizes both HSV-1 gD and HSV-2 gD in the context of class II MHC molecules and has cytolytic potential.
此前已有多篇报道描述了一个令人惊讶的现象，即无法检测到针对单纯疱疹病毒（HSV）重要保护性免疫原之一糖蛋白D（gD）的小鼠细胞毒性T淋巴细胞（CTL）。通过对已发表方法进行细微改动，我们得以证明，感染小鼠对HSV的免疫反应包括产生针对gD的CTL。将纯化的HSV-1注射到C3H/OuJ（H-2k）小鼠的后足垫进行感染。然后分离引流淋巴结中的淋巴细胞，结果显示这些淋巴细胞会对表达HSV-1 gD的转化成纤维细胞（H-2k）产生增殖反应并将其杀死。分离出了两个gD特异性T细胞克隆。其中一个克隆命名为CGD1，显示为CD8+。该克隆在I类主要组织相容性复合体（MHC）分子的背景下识别HSV-1 gD，但不识别HSV-2 gD，并杀死表达HSV-1 gD的合适的MHC匹配的成纤维细胞。这个溶细胞克隆的异常特征包括IL-4增强其对抗原的增殖反应、一种针对Thy-1的单克隆抗体抑制其溶细胞活性以及优先识别感染的成纤维细胞而非感染的淋巴母细胞。另一个克隆命名为CGD3，显示为CD4+。该克隆在II类MHC分子的背景下识别HSV-1 gD和HSV-2 gD，并且具有溶细胞潜力。
