Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA.
J Agric Food Chem. 2009 Oct 14;57(19):9243-9. doi: 10.1021/jf901836f.
Tributyrin, a short-chain triglyceride oil used as a food additive, has been reported to be a potential preventive agent against colon cancer. The purpose of this study was to develop tributyrin delivery systems based on food-grade oil-in-water emulsions that could potentially be incorporated into foods. Emulsions containing only tributyrin as the lipid phase were highly unstable to droplet growth due to Ostwald ripening (OR) because of the relatively high water solubility of this low molecular weight triacylglycerol. The stability of the emulsions to OR could be greatly improved by incorporating >or=15-25% corn oil (a food-grade oil with a low water solubility) into the lipid phase. In addition, the tendency for droplet sedimentation to occur was reduced because the density contrast between the lipid and water phases was reduced in the mixed tributyrin/corn oil systems. The potential anticarcinogenic ability of the tributyrin emulsions was demonstrated using a cell culture model. Treatments with emulsions containing tributyrin significantly inhibited the viability of HT29 colon carcinoma cells. These results have important implications for the development and testing of nutraceuticals encapsulated in food-grade delivery systems as anticancer agents.
丁酸甘油酯，一种作为食品添加剂的短链甘油三酯油，已被报道为预防结肠癌的潜在药物。本研究的目的是开发基于食品级油包水乳液的丁酸甘油酯传递系统，这些系统有可能被纳入食品中。由于奥斯特瓦尔德熟化（OR），仅含有丁酸甘油酯作为脂质相的乳液由于这种低分子量三酰基甘油的相对高水溶性而对液滴生长非常不稳定。通过将>或=15-25%玉米油（一种水溶性低的食品级油）掺入脂质相，可以大大提高乳液对 OR 的稳定性。此外，由于在混合的丁酸甘油酯/玉米油体系中，脂质相与水相之间的密度差异减小，因此减少了液滴沉淀的趋势。通过细胞培养模型证明了丁酸甘油酯乳液的潜在抗癌能力。含有丁酸甘油酯的乳液处理显著抑制了 HT29 结肠癌细胞的活力。这些结果对于开发和测试作为抗癌剂的封装在食品级传递系统中的营养保健品具有重要意义。
