Biomedical Technology Developmental Centre, Fooyin University, Ta-Liao Hsiang, Kaohsiung Hsien, Taiwan.
Clin Microbiol Infect. 2010 Aug;16(8):1090-6. doi: 10.1111/j.1469-0691.2009.03045.x. Epub 2009 Sep 2.
The aim of this study was to explore a gene chip capable of detecting the presence of Mycobacterium tuberculosis isolates directly in clinical sputum specimens and to compare it with current molecular detection techniques. At first, we selected 13 M. tuberculosis-specific target genes to construct a gene chip for rapid diagnosis. Using the membrane array method, we diagnosed M. tuberculosis by gene chip directly from 246 sputum specimens from patients suspected of having tuberculosis. Among 80 M. tuberculosis complex (MTBC) culture-positive sputum specimens, the MTBC detection rate was 62.5% (50/80) by PCR-restriction fragment length polymorphism (RFLP), 70% (56/80) by acid-fast staining, and 85% (68/80) by the membrane array method. Furthermore, subspecies showed different gene expression patterns in the membrane array. In conclusion, MTBC could be detected directly in sputum by the membrane array method. The rapidity of detection and the capability of differentiating subspecies could make this method useful in the control and prevention of tuberculosis.
本研究旨在探索一种能够直接检测临床痰标本中结核分枝杆菌分离株的基因芯片，并将其与当前的分子检测技术进行比较。首先，我们选择了 13 个结核分枝杆菌特异性靶基因，构建了用于快速诊断的基因芯片。我们使用膜阵列法直接从 246 份疑似肺结核患者的痰标本中进行基因芯片诊断结核分枝杆菌。在 80 份结核分枝杆菌复合群（MTBC）培养阳性的痰标本中，PCR-限制性片段长度多态性（RFLP）检测 MTBC 的检出率为 62.5%（50/80），抗酸染色为 70%（56/80），膜阵列法为 85%（68/80）。此外，亚种在膜阵列中表现出不同的基因表达模式。总之，MTBC 可以通过膜阵列法直接从痰液中检测到。该方法检测速度快，能够区分亚种，可用于结核病的控制和预防。
